溶栓治疗对心肌梗死患者Th17及Treg细胞的影响

发布时间：2018-08-24 08:49

【摘要】：目的:观察心肌梗死患者溶栓前后体内Th17、Treg细胞及相关因子含量,从而探讨三者的相关性。方法:提取外周血单个核细胞,流式细胞术检测Th17及Treg含量,ELISA方法检测上清IL-17及TGF-β含量变化,实时定量PCR检测RORγT及Foxp3含量。结果:与对照组相比,实验组患者在溶栓前、后体内Th17细胞,IL-17及RORγT含量均显著升高;Treg细胞、TGF-β及Foxp3含量显著降低(P0.05)。与实验组患者溶栓前相比,溶栓后7 d体内Th17细胞,IL-17及RORγT含量显著降低;Treg细胞、TGF-β及Foxp3含量显著升高(P0.05)。结论:与对照组相比,心肌梗死患者体内中存在Th17及相关因子升高,Treg及相关因子降低,溶栓治疗后可使Th17及相关因子降低,Treg及相关因子升高。
[Abstract]:Aim: to investigate the correlation of Th17,Treg cells and related factors before and after thrombolysis in patients with myocardial infarction. Methods: peripheral blood mononuclear cells were extracted, Th17 and Treg contents were detected by flow cytometry, IL-17 and TGF- 尾 contents in supernatant were detected by Elisa, ROR 纬 T and Foxp3 contents were detected by real-time quantitative PCR. Results: compared with the control group, the contents of IL-17 and ROR 纬 T of Th17 cells in the experimental group were significantly increased before and after thrombolytic therapy (P0.05). The levels of IL-17 and ROR 纬 T in Th17 cells were significantly decreased after 7 days of thrombolysis compared with those before thrombolysis in the experimental group (P0.05). Conclusion: compared with the control group, Th17 and related factors increased and Treg decreased in patients with myocardial infarction. After thrombolytic therapy, Th17 and related factors decreased and Treg and related factors increased after thrombolytic therapy.
【作者单位】： 厦门大学附属第一医院杏林院区内科;厦门大学附属第一医院检验科;厦门大学附属第一医院普外科;厦门大学附属第一医院超声影像科;
【分类号】：R542.22

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