半乳糖凝聚素3对心肌纤维化和心室重构的作用

发布时间：2018-08-24 12:15

【摘要】：目的通过半乳糖凝聚素3(Gal-3)特异性竞争拮抗剂——改良柑橘果胶(MCP)抑制Gal-3途径,探讨Gal-3在心功能不全心室重构中的作用。方法将30只家兔随机分为假手术组、心功能不全组、MCP组。结扎冠状动脉前降支,制作心肌梗死后心功能不全模型。模型制作成功后,MCP组用生理盐水配置75 g/L MCP,以2 m L/(kg·d)灌胃4周;假手术组及心功能不全组给予生理盐水2 m L/(kg·d)灌胃4周。术前及术后2、4、6周,用心脏超声仪测定各组家兔心功能,ELISA检测血清Gal-3、脑钠肽(BNP)水平。实时荧光定量PCR检测心肌组织Gal-3、胶原蛋白Ⅰ、ⅢmRNA的表达,计算Ⅰ/Ⅲ比值。术后6周,Masson染色观察心肌梗死区域组织病理学情况,Western blot检测Gal-3、胶原蛋白Ⅰ、Ⅲ的蛋白表达。结果给药4周后MCP组心功能较心功能不全组明显好转(P0.01)。心功能不全组血清Gal-3高于假手术组(P0.05),MCP组血清Gal-3较心功能不全组明显下降(P0.05)。相关性分析显示,心功能不全组家兔心功能左心室射血分数与血清Gal-3呈负相关性(r=-0.841,P=0.009),左心室舒张末期内径与血清Gal-3呈正相关(r=0.905,P=0.002)。与心功能不全组相比,MCP组Gal-3、胶原蛋白Ⅰ、ⅢmRNA表达明显减低(P0.05)。光学显微镜下心功能不全组可见心肌细胞排列紊乱,部分心肌细胞坏死、水肿,胶原大量沉积并伴有炎性细胞浸润;MCP组可见心肌纤维增粗,排列稍整齐。心功能不全组梗死区域心肌组织Gal-3、胶原蛋白Ⅰ、Ⅲ蛋白表达明显高于MCP组和假手术组;相关性分析显示,心功能不全组梗死区域Gal-3与胶原蛋白Ⅲ呈正相关(r=0.793,P=0.019)。结论 Gal-3在心力衰竭心室重构和心肌纤维化进展中起到关键的作用。
[Abstract]:Objective to investigate the role of Gal-3 in ventricular remodeling by inhibiting Gal-3 pathway by modified citrus pectin (MCP), a specific competitive antagonist of galactose agglutinin 3 (Gal-3). Methods Thirty rabbits were randomly divided into sham operation group and MCP group. The anterior descending coronary artery was ligated to make the model of cardiac insufficiency after myocardial infarction. After the model was successfully made, 75 g / L MCP, was injected into the stomach with saline for 4 weeks, and the sham operation group and cardiac insufficiency group were given saline 2 mL / (kg d) for 4 weeks. The serum levels of Gal-3, brain natriuretic peptide (BNP) were measured by echocardiography and Elisa before operation and 2 weeks after operation. The expression of Gal-3, collagen 鈪，

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