过表达microRNA-486对大鼠心肌梗死后心脏功能损伤的改善

发布时间：2018-08-31 07:23

【摘要】：目的观察直接心肌注射腺病毒搭载micro RNA-486(mi R-486)基因对大鼠心肌梗死后心脏功能损伤的影响。方法SD雄性大鼠,随机分成假手术组(Sham组)、心肌梗死+生理盐水组(MI组)、心肌梗死+空载腺病毒组(Ad组)、心肌梗死+mi R-486腺病毒组(mi R-486组)。于梗死区周围心肌注射不同目标物质。于不同时间点利用实时定量聚合酶链式反应测量各组梗死周边区域mi R-486表达水平。4周时行超声心动图检查,并取各组大鼠心脏行TTC染色,苏木精-依红(HE)染色,马松(Masson)染色,Tunel染色和免疫组化染色。结果随着心肌梗死时间延长mi R-486在3组内的含量有下降的趋势,但mi R-486组mi R-486表达量均较MI组和Ad组明显增加;超声心动图示mi R-486组心功能优于MI组和Ad组;mi R-486组心肌梗死面积较MI组、Ad组明显减少;Masson染色提示mi R-486组、MI组和Ad组胶原容积分数(CVF)明显高于Sham组,但mi R-486组CVF低于MI组和Ad组;MI组和Ad组心肌细胞凋亡指数显著上升但2组间比较差异无统计学意义,mi R-486组与MI组和Ad组相比心肌细胞凋亡指数下降,Sham组未见明显心肌细胞凋亡;mi R-486组微血管密度(MVD)显著高于Sham组、MI组和Ad组,且MI组、Ad组心肌MVD显著高于Sham组。结论 mi R-486的心肌注射可通过抑制大鼠心肌梗死后纤维化,减少心肌细胞凋亡和促进缺血区域微血管新生,进而对心肌梗死后心脏功能有所改善。
[Abstract]:Objective to investigate the effects of micro RNA-486 (mi R-486) gene on cardiac function injury after myocardial infarction in rats. Methods male SD rats were randomly divided into three groups: sham operation group (Sham group), myocardial infarction saline group (MI group), myocardial infarction non-loaded adenovirus group (Ad group) and myocardial infarction mi R-486 adenovirus group (mi R-486 group). Different target substances were injected into the myocardium around the infarct area. At different time points, real-time quantitative polymerase chain reaction was used to measure the expression level of mi R-486 in the periinfarction area of each group. Echocardiography was performed at 4 weeks. The heart of each group was stained with TTC and hematoxylin / eosin (HE) staining. Ma Song (Masson) staining and immunohistochemical staining. Results with the prolongation of myocardial infarction time, the content of mi R-486 decreased in three groups, but the expression of mi R-486 in mi R-486 group was significantly higher than that in MI group and Ad group. Echocardiography showed that the cardiac function of mi R-486 group was better than that of MI group and Ad group. The myocardial infarction size of mi R-486 group was significantly lower than that of MI Ad group. The collagen volume fraction (CVF) of mi R-486 group in MI group and Ad group was significantly higher than that of Sham group. But CVF of mi R-486 group was lower than that of MI group and Ad group. Myocardial apoptosis index in MI group and Ad group was significantly increased, but there was no significant difference between the two groups. There was no significant decrease in myocardial apoptosis index between MI group and Ad group. The microvessel density (MVD) in myocardial apoptosis group was significantly higher than that in Sham group (MI group) and Ad group (P < 0.05). Myocardial MVD in Ad group was significantly higher than that in Sham group. Conclusion Myocardial injection of mi R-486 can inhibit myocardial fibrosis, decrease cardiomyocyte apoptosis and promote angiogenesis in ischemic area, and then improve cardiac function after myocardial infarction.
【作者单位】：山东省济宁市第一人民医院心内科;承德医学院附属医院心外科;北京同仁医院心外科;海军总医院心内科;
【基金】：国家自然科学基金(81370237)
【分类号】：R542.22

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