拟交感效应对快速起搏离体心脏心房缝隙连接蛋白43重构的影响

发布时间：2018-09-14 19:40

【摘要】：目的:通过建立离体拟交感心房颤动模型,探讨缝隙连接蛋白43(Cx43)水平的变化。方法:15只杂种犬随机分为3组(每组5只):对照组(control组)、快速心房起搏组(RAP组)和异丙肾上腺素灌流+快速心房起搏组(ISO+RAP组)。经胸骨正中切开术,快速取出心脏,建立心脏Langendorff离体灌流模型。各组分别检测心房有效不应期(AERP)及房颤诱发率,免疫组化检测酪氨酸羟化酶(TH)在细胞内的表达,Western blot检测Cx43和磷酸化Cx43的蛋白含量,免疫荧光检测Cx43在心肌组织的变化,TUNEL法检测心肌细胞凋亡,荧光比色法检测线粒体活性氧簇(ROS)的生成量。结果:与control组比较,RAP组的AERP无明显变化,且仅出现电紊乱,ISO+RAP组的AERP明显缩短(P0.05),并可成功诱发房颤。与control组比较,RAP组和ISO+RAP组的TH表达量、细胞凋亡指数和线粒体ROS生成量均逐渐增多(P0.05),Cx43蛋白表达量和磷酸化水平逐渐减少(P0.05)。免疫荧光显示,与control组相比,RAP组的Cx43荧光强度降低,且呈明显侧链化,ISO+RAP组则呈点状散在分布。结论:交感神经可能通过氧化应激效应,引起Cx43的重构与下调,从而介导心房颤动的发生。
[Abstract]:AIM: To investigate the changes of connexin 43 (Cx43) level in isolated sympathetic atrial fibrillation (PSAF) model. Methods: Fifteen dogs were randomly divided into three groups: control group, rapid atrial pacing group (RAP) and isoprenaline perfusion + rapid atrial pacing group (ISO + RAP). The effective refractory period (AERP) and the induction rate of atrial fibrillation were detected, the expression of tyrosine hydroxylase (TH) was detected by immunohistochemistry, the contents of Cx43 and phosphorylated Cx43 were detected by Western blot, the changes of Cx43 in myocardial tissue were detected by immunofluorescence, and the myocardium was detected by TUNEL. Results: Compared with the control group, the AERP of RAP group showed no significant change, and only electrical disturbance. The AERP of ISO + RAP group was significantly shortened (P 0.05), and AF could be induced successfully. Compared with the control group, the TH expression, apoptosis index and mitochondrial RO of RAP group and ISO + RAP group were detected. S production increased gradually (P 0.05), Cx43 protein expression and phosphorylation level decreased gradually (P 0.05). Immunofluorescence showed that Cx43 fluorescence intensity in RAP group was lower than that in control group, and the Cx43 fluorescence intensity was obviously side chain, while that in ISO + RAP group was mainly distributed. It can mediate the occurrence of atrial fibrillation.
【作者单位】：广西医科大学第一附属医院老年心血管内科;广西医科大学第一附属医院心电诊断科;广西医科大学第一附属医院心血管病研究所;
【分类号】：R541.7;;R363

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