比索洛尔对心力衰竭大鼠心肌肌浆网钙ATP酶2a活性的影响

发布时间：2018-09-19 18:42

【摘要】：目的:观察比索洛尔对心力衰竭大鼠心肌肌浆网钙ATP酶2a(SERCA2a)活性的影响。方法:采用阿霉素腹腔注射法建立大鼠心力衰竭模型,随机分为正常对照组、假手术组、模型组、比索洛尔组、卡托普利组和比索洛尔+卡托普利组,分别以蒸馏水、比索洛尔、卡托普利、比索洛尔+卡托普利连续灌胃35d。观察大鼠心功能指标,ELISA法检测血浆脑钠肽水平,茎环状引物实时定量PCR检测心肌miR-25-3p表达水平,Western blot检测心肌SERCA2a和PLB蛋白表达水平,定磷法测定心肌SERCA2a活性。结果:比索洛尔组、卡托普利组和比索洛尔+卡托普利组大鼠心功能指标明显高于模型组,血浆脑钠肽水平明显低于模型组,miR-25-3p表达水平明显低于模型组,SERCA2a和受磷蛋白(PLB)蛋白表达水平明显高于模型组(P0.01)。比索洛尔组和比索洛尔+卡托普利组SERCA2a/PLB比值和SERCA2a活性明显高于模型组(P0.01或P0.05),卡托普利组与模型组比较无明显差异。结论:比索洛尔可以改善心力衰竭大鼠心功能,机制可能与下调心肌miR-25-3p表达水平,提高SERCA2a、PLB表达水平,提升SERCA2a/PLB比值,增强SERCA2a活性有关。
[Abstract]:Aim: to observe the effect of bisoprolol on the activity of calcium ATP enzyme 2a (SERCA2a) in cardiac sarcoplasmic reticulum in rats with heart failure. Methods: rat heart failure model was established by intraperitoneal injection of doxorubicin. The rats were randomly divided into normal control group, sham operation group, model group, Bisoprolol group, captopril group and Bisoprotopril group, with distilled water. Bisoprolol, captopril, Bisoprolol captopril were perfused by stomach for 35 days. The plasma brain natriuretic peptide (BNP) levels were detected by Elisa, miR-25-3p expression level was detected by real-time quantitative PCR with stem ring primer, the expression of SERCA2a and PLB protein was detected by Western blot, and the activity of SERCA2a was measured by phosphorous determination method. Results: the cardiac function indexes of rats in the Bisoprolol group, captopril group and Bisoprotopril group were significantly higher than those in the model group. The level of plasma brain natriuretic peptide (BNP) was significantly lower than that of the model group (P0.01). The level of plasma brain natriuretic peptide (BNP) was significantly lower than that of the model group (P0.01). The ratio of SERCA2a/PLB and the activity of SERCA2a were significantly higher in Bisoprolol group and Bisoprotopril group than in model group (P0.01 or P0.05), but there was no significant difference between captopril group and model group. Conclusion: bisoprolol can improve cardiac function in rats with heart failure. The mechanism may be related to the down-regulation of myocardial miR-25-3p expression, the increase of SERCA2a,PLB expression, the increase of SERCA2a/PLB ratio and the enhancement of SERCA2a activity.
【作者单位】：广州中医药大学博士后流动站;东莞市中医院心内科;广州中医药大学第一附属医院心内科;
【分类号】：R541.6

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