ADAM17调控CGRP对血管紧张素Ⅱ诱导的血管平滑肌细胞增殖的抑制效应
发布时间：2018-10-11 18:21
【摘要】：目的阐明肿瘤坏死因子α转化酶(ADAM17)调控降钙素基因相关肽(CGRP)的抗血管平滑肌细胞(VSMC)增殖效应。方法以大鼠源性胸主动脉平滑肌细胞株(A10VSMC)为研究对象,采用RNA干扰抑制ADAM17表达;采用蛋白质免疫印迹和实时定量PCR检测蛋白和mRNA表达水平;采用噻唑蓝比色法评估细胞活性。结果 CGRP显著抑制血管紧张素Ⅱ介导的VSMC增殖。血管紧张素Ⅱ刺激24 h后,VSMC中ADAM17表达显著增加。CGRP预处理30 min能显著降低VSMC中ADAM17表达,而CGRP受体拮抗剂CGRP8-37可抑制或逆转上述效应。ADAM17 RNA干扰可显著抑制血管紧张素Ⅱ介导的VSMC增殖。结论降低ADAM17表达可能是CGRP抑制血管紧张素Ⅱ介导的VSMC增殖效应的机制。
[Abstract]:Aim to elucidate the effect of tumor necrosis factor alpha converting enzyme (ADAM17) on the proliferation of vascular smooth muscle cells (VSMCs) regulated by calcitonin gene-related peptide (CGRP). Methods Rat thoracic aortic smooth muscle cell line (A10VSMC) was used to inhibit ADAM17 expression by RNA interference, protein and mRNA expression levels were detected by Western blot and real-time quantitative PCR, and cell activity was evaluated by thiazolyl blue colorimetry. Results CGRP significantly inhibited angiotensin 鈪，
本文编号：2264795
[Abstract]:Aim to elucidate the effect of tumor necrosis factor alpha converting enzyme (ADAM17) on the proliferation of vascular smooth muscle cells (VSMCs) regulated by calcitonin gene-related peptide (CGRP). Methods Rat thoracic aortic smooth muscle cell line (A10VSMC) was used to inhibit ADAM17 expression by RNA interference, protein and mRNA expression levels were detected by Western blot and real-time quantitative PCR, and cell activity was evaluated by thiazolyl blue colorimetry. Results CGRP significantly inhibited angiotensin 鈪，
本文编号：2264795
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