缬沙坦对球囊损伤大鼠主动脉内皮后血凝素样氧化低密度脂蛋白受体-1表达的影响

发布时间：2018-10-12 10:49

【摘要】：目的:通过观察血管内皮损伤后内膜增生过程中血凝素样氧化低密度脂蛋白受体-1表达的变化,探讨血凝素样氧化低密度脂蛋白受体-1(LOX-1)在再狭窄中的作用机制及缬沙坦对其作用。方法:建立球囊损伤大鼠主动脉内皮模型,并将大鼠随机分为对照组、损伤组、药物组,每组分别于术后14天、28天取动脉组织,行HE染色观察血管内膜增生的过程,逆转录聚合酶链反应及免疫组化技术检测主动脉组织内血凝素样氧化低密度脂蛋白受体-1的m RNA及蛋白的表达。结果:术后14及28d损伤组大鼠主动脉血管内膜较对照组均显著增厚(P0.05),术后l4及28d缬沙坦药物组内膜增生程度均轻于损伤组(P0.05),术后14及28d,损伤组血凝素样氧化低密度脂蛋白受体-1m RNA及蛋白表达较对照组均显著升高(P0.05),药物组血凝素样氧化低密度脂蛋白受体-1m RNA及蛋白表达较损伤组降低(P0.05)。结论:大鼠主动脉内皮损伤后,血管内膜增生,血凝素样氧化低密度脂蛋白受体-1表达增高;缬沙坦可减少血凝素样氧化低密度脂蛋白受体-1的表达,减轻损伤血管内膜增生的程度。
[Abstract]:Aim: to observe the changes of hemagglutinin like oxidized low density lipoprotein receptor-1 during intimal hyperplasia after vascular endothelial injury. To investigate the mechanism of hemagglutinin-like oxidation of low density lipoprotein receptor-1 (LOX-1) in restenosis and the effect of valsartan on it. Methods: the aortic endothelial model was established in rats with balloon injury. The rats were randomly divided into three groups: control group, injury group and drug group. Arterial tissues were taken from each group on the 14th and 28th days after operation, and the process of intimal hyperplasia was observed by HE staining. Reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical technique were used to detect the expression of m RNA and protein of hemagglutinin like oxidized low density lipoprotein receptor-1 in aorta. Results: the intima of aorta in the injury group was significantly thicker than that in the control group on the 14th and 28th days after operation (P0.05), and the intimal hyperplasia degree of valsartan drug group was lighter than that of the injured group on the 4th and 28th days after operation (P0.05). On the 14th and 28th days after operation, the hemagglutinin-like oxidation in the injured group was lower than that in the control group (P0.05). The expression of lipoprotein receptor-1m RNA and protein was significantly higher than that of control group (P0.05), and the expression of hemagglutinin like oxidized low density lipoprotein receptor-1m RNA and protein in drug group was lower than that in injured group (P0.05). Conclusion: after aortic endothelial injury, vascular intima proliferates, hemagglutinin like oxidized low density lipoprotein receptor-1 expression is increased, valsartan can reduce the expression of hemagglutinin like oxidized low density lipoprotein receptor 1, valsartan can reduce the expression of hemagglutinin like oxidized low density lipoprotein receptor 1. Reduce the degree of intimal hyperplasia of injured vessels.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R541.4

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