甜菜碱对高SAH诱导动脉粥样硬化保护作用

发布时间：2018-10-19 09:55

【摘要】：目的探讨甜菜碱补充对高S-腺苷同型半胱氨酸(SAH)诱导的动脉粥样硬化(AS)的影响。方法采用apo E基因敲除(apoE~(-/-))小鼠制备AS模型,饮食添加高蛋氨酸和SAH水解酶抑制剂建立高SAH模型,在SAH模型基础上饮食添加不同剂量甜菜碱干预,观察小鼠血脂水平、血浆SAH、S-腺苷蛋氨酸(SAM)、同型半胱氨酸(Hcy)浓度以及SAM/SAH比值变化,采用油红O染色观察apoE~(-/-)小鼠主动脉窦AS斑块情况。结果与对照组比较,高SAH模型组小鼠血浆SAH水平[(78.61±4.62)nmol/L]升高,SAM水平[(40.17±3.02)nmol/L]及SAM/SAH比值(0.65±0.11)降低,Hcy水平[(6.97±0.51)μmol/L]无明显变化,AS斑块绝对面积[(52.65±8.40)mm~2]和相对面积(25.47±5.23)%均增加;与高SAH模型组比较,高剂量甜菜碱组小鼠血浆SAH水平[(30.18±3.85)nmol/L]、Hcy水平[(4.91±0.30)μmol/L]降低,SAM水平[(78.14±3.99)nmol/L]及SAM/SAH比值(2.78±0.34)升高,AS斑块绝对和相对面积[(28.75±7.24)mm~2、(13.89±3.96)%]下降;呈剂量-效应关系。结论甜菜碱干预可降低apoE~(-/-)小鼠血浆高SAH水平,对高SAH诱导的动脉粥样硬化具有缓解作用。
[Abstract]:Objective to investigate the effect of betaine supplementation on atherosclerotic (AS) induced by high S-adenosine homocysteine (SAH). Methods apo E gene knockout (apoE~ (- / -) mice were used to make AS model. High SAH model was established by adding high methionine and SAH hydrolase inhibitor into diet. On the basis of SAH model, different doses of betaine were added to the model to observe the level of blood lipid in mice. The changes of plasma adenosine methionine (SAM), homocysteine (Hcy) and SAM/SAH ratio were studied. AS plaques in the aortic sinus of apoE~ (- / -) mice were observed by oil red O staining. Results compared with the control group, the plasma SAH level [(78.61 卤4.62) nmol/L], the SAM level [(40.17 卤3.02) nmol/L] and the SAM/SAH ratio (0.65 卤0.11) in the high SAH model group were increased, the Hcy level [(6.97 卤0.51) 渭 mol/L] had no significant change, and the absolute area of AS plaque [(52.65 卤8.40) mm~2] and the relative area (25.47 卤5.23)% in the high SAH model group. Plasma SAH level [(30.18 卤3.85) nmol/L], Hcy level [(4.91 卤0.30) 渭 mol/L] decreased, SAM level [(78.14 卤3.99) nmol/L] and SAM/SAH ratio (2.78 卤0.34) increased, and absolute and relative area of AS plaque [(28.75 卤7.24) mm~2, (13.89 卤3.96)%] decreased in a dose-dependent manner. Conclusion Betaine intervention can reduce the level of high SAH in plasma of apoE~ (- / -) mice and alleviate atherosclerosis induced by high SAH.
【作者单位】：深圳市疾病预防控制中心;中山大学公共卫生学院;
【基金】：国家自然科学基金(81402672)
【分类号】：R543.5

【相似文献】