抑制干扰素诱导蛋白16表达对人脑血管外膜成纤维细胞增殖、凋亡及迁移的影响

发布时间：2018-10-22 06:51

【摘要】：目的探讨抑制干扰素诱导蛋白16(IFI16)表达后对人脑血管外膜成纤维细胞(HBVAF)增殖、凋亡与迁移的影响及其可能机制。方法培养HBVAF分3组,未经处理的HBVAF作为空白对照组,转染非特异性小干扰RNA(siRNA)的HBVAF作为阴性对照组,转染IFI16siRNA的HBVAF作为IFI16siRNA组。应用蛋白免疫印迹和实时荧光定量聚合酶链反应(PCR)测定细胞中IFI16、p53、p21蛋白和mRNA表达水平。用四甲基偶氮唑盐(MTT)比色法检测细胞活力,流式细胞术测定细胞凋亡,Transwell法测定细胞迁移情况。结果与阴性对照组比较,转染IFI16siRNA后,HBVAF中IFI16、p53及p21蛋白和mRNA表达水平下调,IFI16siRNA组MTT吸光度值增高(0.70±0.01比0.65±0.01,P0.05),IFI16siRNA组、阴性对照组与空白对照组之间在细胞迁移数目与凋亡比例上差异无统计学意义。结论抑制IFI16表达可促进HBVAF增殖,其机制可能部分与抑制p53及p21表达有关。
[Abstract]:Objective to investigate the effect of inhibiting the expression of interferon inducible protein 16 (IFI16) on the proliferation, apoptosis and migration of human cerebrovascular adventitial fibroblasts (HBVAF) and its possible mechanism. Methods HBVAF were divided into three groups: untreated HBVAF as blank control group, HBVAF transfected with non-specific small interfering RNA (siRNA) as negative control group and IFI16siRNA HBVAF as IFI16siRNA group. Western blot and real-time fluorescence quantitative polymerase chain reaction (PCR) were used to detect the expression of IFI16,p53,p21 protein and mRNA. Cell viability was detected by (MTT) assay, apoptosis was detected by flow cytometry, and cell migration was measured by Transwell assay. Results compared with the negative control group, the expression of IFI16,p53 and p21 protein and mRNA in HBVAF were down-regulated after transfection of IFI16siRNA, and the absorbance of MTT in IFI16siRNA group was increased (0.70 卤0.01 vs 0.65 卤0.01 P 0.05). There was no significant difference in the number of cell migration and the proportion of apoptosis between the negative control group and the blank control group. Conclusion inhibiting the expression of IFI16 can promote the proliferation of HBVAF, and the mechanism may be related to the inhibition of p53 and p21 expression.
【作者单位】：贵州省人民医院心内科;
【基金】：国家自然科学基金项目(81260030)
【分类号】：R54

【相似文献】