人冠状动脉粥样硬化病灶内CD40L水平与病灶结构变化的关系

发布时间：2018-10-22 14:24

【摘要】：目的观察人冠状动脉粥样硬化病灶结构变化、CD40L、基质金属蛋白酶9(MMP-9)蛋白表达水平,探讨CD40/CD40L信号在人冠状动脉粥样硬化发生发展中的作用。方法收集不同程度粥样硬化的人冠状动脉血管60例作为实验组,以12例无病变的人冠状动脉为对照组,常规HE染色观察两组人群冠状动脉的组织结构并用图像分析软件检测病灶结构的相关指标,免疫组织化学染色检测CD40L、MMP-9蛋白表达水平,分析CD40L、MMP-9表达水平与动脉硬化病灶结构变化之间的关系。结果发生粥样硬化的冠状动脉CD40L表达均增强,其表达主要分布于斑块肩部和底部的泡沫细胞。同时病灶内MMP-9表达水平增高并与CD40L水平、病灶大小尤其是坏死灶的大小呈正相关,MMP-9表达水平与纤维帽厚度无明显相关性。随着MMP-9水平增高,纤维帽厚度/内膜最大厚度比值降低。结论人冠状动脉粥样硬化病灶内CD40L、MMP-9表达水平明显增强,CD40L可能通过促进MMP-9表达,使血管病灶内细胞外基质分解加强,从而促进病变的进展及坏死灶的扩大,使粥样病灶稳定性下降。
[Abstract]:Objective to investigate the changes of lesion structure and the expression of CD40L, matrix metalloproteinase-9 (MMP-9) protein in human coronary atherosclerosis, and to explore the role of CD40/CD40L signal in the pathogenesis and development of human coronary atherosclerosis. Methods Sixty patients with different degree of atherosclerosis were selected as experimental group and 12 cases as control group. The tissue structure of coronary artery in both groups was observed by routine HE staining and the relative indexes of lesion structure were detected by image analysis software. The expression level of CD40L,MMP-9 protein was detected by immunohistochemical staining. To analyze the relationship between the expression of CD40L,MMP-9 and the structural changes of arteriosclerosis lesions. Results the expression of CD40L was increased in the coronary artery with atherosclerosis, and the expression was mainly distributed in foam cells in the shoulder and bottom of the plaque. At the same time, there was a positive correlation between the expression of MMP-9 and CD40L, the size of lesion, especially the size of necrotic foci, but there was no significant correlation between the expression of MMP-9 and the thickness of fibrous cap. With the increase of MMP-9 level, the ratio of fiber cap thickness to maximum intimal thickness decreased. Conclusion the expression of CD40L,MMP-9 in human coronary atherosclerotic lesions is significantly increased, and CD40L may promote the development of the lesions and the expansion of necrotic foci by promoting the expression of MMP-9 and enhancing the decomposition of extracellular matrix in the lesions. The stability of atherosclerotic lesion was decreased.
【作者单位】：贵州医科大学病理学教研室;贵州医科大学法医学教研室;贵州医科大学法医司法鉴定中心;
【基金】：贵州省高校工程技术研究中心建设项目(黔科合KY字[2013]114)
【分类号】：R541.4

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