p15、DAPK、SOCS1和FHIT 4基因甲基化联合检测在骨髓增生异常综合征早期诊断及预后评估中的价值

发布时间：2018-10-23 14:44

【摘要】：目的:探讨p15、DAPK、SOCS1和FHIT 4基因甲基化联合检测在骨髓增生异常综合征(MDS)早期诊断与预后评估中的价值。方法:应用甲基化特异性PCR(MSP)对67例MDS患者骨髓进行上述4种基因甲基化检测,分析4个基因联检在MDS患者早期诊断及预后评估中的价值。结果:67例MDS患者p15、DAPK、SOCS1和FHIT 4个基因甲基化率分别为37.3%、35.8%、47.8%和52.2%,较对照组显著增高(P0.05),4个基因单检诊断MDS的阳性符合率分别为37.3%、35.8%、47.8%和52.2%,而4个基因联合检测诊断MDS的阳性符合率为82.1%,较单一基因检查阳性符合率明显增高(P0.001);相对高危组中≥2个基因甲基化共表达明显高于相对低危组(P0.05)。MDS患者中位生存时间18(13.3,22.7)个月,相对低危组患者中位生存时间明显长于相对高危组[27(20.3,33.7)vs 9(5.9,12.1)个月](P0.05)。在不同危险度患者中,随着表达基因数的增多,患者的生存时间呈明显缩短趋势(P0.05)。结论:p15、DAPK、SOCS1和FHIT 4种基因联合检测有利于提高MDS患者的早期诊断和预后判断。
[Abstract]:Objective: to investigate the value of combined detection of methylation of p15 DAPKG SOCS1 and FHIT 4 gene in the early diagnosis and prognosis of myelodysplastic syndrome (MDS). Methods: methylation specific PCR (MSP) was used to detect the methylation of bone marrow of 67 patients with MDS, and the value of combined detection of four genes in early diagnosis and prognosis evaluation of MDS patients was analyzed. Results: the methylation rates of p15DAPKG SOCS1 and FHIT in 67 patients with MDS were 37.335.8% and 52.2%, respectively, which were significantly higher than those in the control group (P0.05). The positive coincidence rates of the four genes in the diagnosis of MDS were 37.335.8% and 52.2%, respectively, and the positive coincidence rates of the four genes combined in the diagnosis of MDS were 37.3% and 52.2%, respectively. The positive coexpression rate of more than 2 genes in relative high risk group was significantly higher than that in relatively low risk group (P0.05). The median survival time of). MDS patients was 18 months (13.322.7 months). The median survival time of the patients in the relatively low risk group was significantly longer than that in the relatively high risk group [27 (20.3 / 33.7) vs 9 (5.9 / 12.1) months] (P0.05). In patients with different risk, with the increase of the number of expressed genes, the survival time of patients was significantly shortened (P0.05). Conclusion: the combined detection of p15 DAPKG SOCS1 and FHIT genes is helpful to improve the early diagnosis and prognosis of MDS patients.
【作者单位】：新疆医科大学第一附属医院血液病中心;
【基金】：新疆维吾尔自治区自然科学基因(2015211C046)
【分类号】：R551.3

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