二甲双胍对饱和脂肪酸诱导的大鼠H9C2型心肌细胞损伤的保护作用研究

发布时间：2018-10-23 18:43

【摘要】：目的探讨二甲双胍(Met)对饱和脂肪酸所诱导的大鼠H9C2型心肌细胞损伤作用的保护机制。方法在对照、棕榈酸(PA)及3种不同浓度梯度Met与PA联合组培养液中培养大鼠H9C2型心肌细胞株24h。采用蛋白质印迹法(Western blot)测定各组细胞核因子κB(NF-κB)p65、细胞间黏附因子(ICAM1)、磷酸化核因子κB抑制蛋白(p-IκBα)及磷酸化腺苷酸活化蛋白激酶(p-AMPK)的蛋白表达,实时荧光定量PCR测定各组细胞NF-κB、单核细胞趋化因子(CCL2)和ICAM1的mRNA表达。结果与对照组相比,PA组的细胞中NF-κB p65、ICAM1、p-IκBα蛋白表达量增加(P0.05);与PA组相比,Met+PA联合组细胞NF-κB p65、ICAM1、p-IκBα的蛋白表达量随Met浓度梯度增加表现为不同程度递减(P0.05),p-AMPK蛋白表达量随Met浓度增加而显著增加(P0.05)。与对照组相比,PA组CCL2、ICAM1的mRNA表达量增加(P0.05);与PA组相比,Met+PA联合组细胞CCL2和ICAM1的mRNA表达量下降(P0.05)。结论 Met可以减轻由饱和脂肪酸诱导细胞黏附因子和趋化因子表达增加而引起的大鼠H9C2型心肌细胞损伤。
[Abstract]:Objective to investigate the protective mechanism of metformin (Met) on H9C2 type cardiomyocyte injury induced by saturated fatty acids in rats. Methods Rat cardiomyocytes of H9C2 type were cultured in the culture medium of palmitic acid (PA) and three different concentration gradient Met and PA for 24 h. The protein expressions of nuclear factor 魏 B (NF- 魏 B) p65, intercellular adhesion factor (ICAM1), phosphorylated nuclear factor 魏 B inhibitor protein (p-I 魏 B 伪) and phosphorylated adenylate activated protein kinase (p-AMPK) were detected by Western blot (Western blot). The mRNA expression of NF- 魏 B, monocyte chemokine (CCL2) and ICAM1 were measured by real-time fluorescence quantitative PCR. Results compared with the control group, the expression of NF- 魏 B p65, ICAM1, p-I 魏 B 伪 protein in PA group was increased (P0.05), and the expression of NF- 魏 B p65 ICAM1 p-I 魏 B 伪 protein in, Met PA combined group was decreased in varying degrees with the increase of Met concentration gradient (P0.05), and p-AMPK protein expression level decreased with Met concentration gradient (P0.05). The degree increased significantly (P0.05). Compared with the control group, the mRNA expression of CCL2,ICAM1 in PA group was increased (P0.05), and the mRNA expression of CCL2 and ICAM1 in, Met PA combined group was lower than that in PA group (P0.05). Conclusion Met can attenuate the damage of H9C2 type cardiomyocytes induced by saturated fatty acid induced increased expression of adhesion factor and chemokine in rat cardiomyocytes.
【作者单位】：贵州医科大学附属医院心血管内科;济南军区总医院;
【基金】：全军后勤科研重大项目(AWS13C008)
【分类号】：R54

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