HDAC8在异丙肾上腺素诱导大鼠心肌纤维化中的作用

发布时间：2018-10-30 15:10

【摘要】：目的初步探讨组蛋白去乙酰化酶(HDAC8)在盐酸异丙肾上腺素(ISO)诱导的大鼠心肌纤维化中表达的机制。方法将40只雄性SD大鼠随机均等分为正常组和模型组。模型组给予ISO 1次/d(5 mg/kg),正常组予以等剂量生理盐水皮下注射,连续注射7 d后处死大鼠取血液标本,并取心肌组织。测定心脏质量指数;ELISA法检测血清中I型胶原和Ⅲ型胶原的含量;HE和Masson染色法观察心肌纤维化程度;Western blot法测定HDAC8和α-平滑肌肌动蛋白(α-SMA)蛋白表达情况;qRT-PCR法检测HDAC8 mRNA的表达。结果与正常组比较,模型组心脏质量指数明显增加(P0.05);HE和Masson染色显示模型组心肌组织出现明显的胶原纤维增生;模型组血清标本中Ⅰ型胶原和Ⅲ型胶原的含量较正常组明显增加(P0.05);Western blot法检测显示模型组HDAC8、α-SMA的蛋白表达明显高于正常组(P0.05,P0.01);qRT-PCR检测模型组HDAC8 mRNA表达明显高于正常组(P0.05)。结论 HDAC8在心肌纤维化中高表达,而在正常心肌组织中低表达,提示其在心肌纤维化的发生发展中可能发挥作用。
[Abstract]:Objective to investigate the mechanism of histone deacetylase (HDAC8) expression in myocardial fibrosis induced by isoproterenol (ISO) in rats. Methods 40 male SD rats were randomly divided into normal group and model group. The model group was given ISO once a day (5 mg/kg), and the normal group was subcutaneously injected with the same dose of normal saline. After 7 days of continuous injection, the rats were sacrificed to get blood samples and myocardial tissue. Cardiac mass index, serum type I collagen and type 鈪，

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