microRNA-150上调可改善心肌梗死后心肌纤维化及心功能

发布时间：2018-10-30 14:59

【摘要】：目的急性心肌梗死是世界发病率及致死率最高的疾病之一。而心肌梗死后心肌纤维化是急性心肌梗死患者心功能不全的重要机制。微小RNA是一种非编码RNA,可调控基因表达,与急性心肌梗死密切相关。本文旨在探讨microRNA-150在心肌梗死大鼠的心肌组织中的表达,并进一步研究miR-150对心肌梗死后心肌纤维化及心脏功能的作用。方法通过结扎Sprague Dawley大鼠心脏冠状动脉左前降支构造心肌梗死模型,采用过表达microRNA-150的慢病毒上调大鼠心肌组织中的miR-150表达水平,进一步通过RT-PCR检测心肌梗死边缘区microRNA-150的表达及col1ɑ1和α-SMA的信使RNA的含量,通过Western Blotting检测心肌梗死边缘区col1ɑ1、α-SMA蛋白的水平,通过心脏彩超检查及Masson染色分别检测心脏功能及心肌纤维化,研究microRNA-150在心肌梗死大鼠心肌组织中的表达及其对心肌梗死后纤维化及心脏功能的影响。结果实验结果表明microRNA-150在心肌梗死术后第28天梗死边缘区心肌中表达降低(P0.05)。此外,上调心肌梗死大鼠心肌组织中miRNA-150的表达水平可改善心肌梗死后心功能,降低胶原容积分数并且降低心肌组织中col1ɑ1及ɑ-SMA的表达(P0.001)结论MicroRNA-150在心肌梗死边缘区心肌组织中表达是下降的,而上调心肌组织中microRNA-150的表达可抑制心肌梗死后纤维化并改善心脏功能。
[Abstract]:Objective Acute myocardial infarction (AMI) is one of the diseases with the highest morbidity and mortality in the world. Myocardial fibrosis after myocardial infarction is an important mechanism of cardiac insufficiency in patients with acute myocardial infarction. Micro RNA is a non-coding RNA, regulated gene expression, which is closely related to acute myocardial infarction. The purpose of this study was to investigate the expression of microRNA-150 in myocardial tissue of rats with myocardial infarction and to further study the effects of miR-150 on myocardial fibrosis and cardiac function after myocardial infarction. Methods the myocardial infarction model was constructed by ligating the left anterior descending branch of coronary artery in Sprague Dawley rats. The lentivirus overexpression of microRNA-150 was used to up-regulate the expression of miR-150 in myocardial tissue of rats. Furthermore, the expression of microRNA-150 and the content of col1 1 and 伪-SMA messenger RNA were detected by RT-PCR, and the levels of col1 1, 伪-SMA protein were detected by Western Blotting. Cardiac function and myocardial fibrosis were detected by color Doppler echocardiography and Masson staining. The expression of microRNA-150 in myocardial tissue of rats with myocardial infarction and its effect on myocardial fibrosis and cardiac function after myocardial infarction were studied. Results the results showed that the expression of microRNA-150 in the myocardium of myocardial infarction margin was decreased on the 28th day after myocardial infarction (P0.05). In addition, upregulating the expression of miRNA-150 in myocardial tissue of myocardial infarction rats can improve the cardiac function after myocardial infarction. Decrease of collagen volume fraction and expression of col1 1 and SMA in myocardial tissue (P0. 001) conclusion the expression of MicroRNA-150 in myocardial tissue of myocardial infarction marginal area is decreased. Upregulating the expression of microRNA-150 in myocardial tissue can inhibit fibrosis and improve cardiac function after myocardial infarction.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R542.22

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