依普利酮部分逆转甲状腺激素诱导的心肌电重构

发布时间：2018-11-17 08:35

【摘要】：目的:研究醛固酮拮抗剂依普利酮对甲状腺激素(T3)诱导的心肌电重构的影响。方法:原代培养SD乳鼠心肌细胞,并随机分为对照组、T3组、依普利酮(Epl)组、T3+Epl组采用免疫荧光法鉴定心肌细胞,CCK-8法检测T3与依普利酮对心肌细胞存活率的影响,细胞免疫荧光法、real-time PCR法、Western blot法检测各组钾离子通道Kv1.5、Kv4.3,L型钙离子通道Cav1.2缝隙连接蛋白40(Cx40)和Cx43 mRNA与蛋白的表达。结果:免疫荧光法显示所培养细胞为心肌细胞且95%以上横纹肌α-辅肌动蛋白(sarcomericα-actinin,α-actinin)抗体染色阳性。与对照组比较,T3增加Kv1.5、Kv4.3、Cav1.2与Cx40 mRNA与蛋白的表达,减少Cx43 mRNA与蛋白的表达;Ep1组Kv1.5、Kv4.3、Cav1.2与Cx40 mRNA与蛋白的表达降低,Cx43的表达升高;与T3组比较,T3+Epl组Kv1.5、Kv4.3、Cav1.2与Cx40 mRNA与蛋白的表达降低,Cx43的表达升高。结论:依普利酮可部分逆转甲状腺激素导致的心肌电重构。
[Abstract]:Aim: to study the effect of aldosterone antagonist epirone on myocardial electrical remodeling induced by thyroid hormone (T 3). Methods: primary cultured SD neonatal rat cardiomyocytes were randomly divided into three groups: control group, T3 group, (Epl) group, T3 Epl group, immunofluorescence assay. The effects of T3 and Epridone on the survival rate of cardiomyocytes were detected by CCK-8. The expression of Cav1.2 gap junction protein 40 (Cx40), Cx43 mRNA and protein in potassium channel Kv1.5,Kv4.3,L type calcium channel Cav1.2 were detected by cell immunofluorescence assay and real-time PCR, Western blot method. Results: immunofluorescence assay showed that the cultured cells were cardiomyocytes and more than 95% of the striated muscle 伪 -actin (sarcomeric 伪-actinin, 伪-actinin) antibodies were positive. Compared with the control group, T3 increased the expression of Kv1.5,Kv4.3,Cav1.2 and Cx40 mRNA and protein, decreased the expression of Cx43 mRNA and protein, decreased the expression of Kv1.5,Kv4.3,Cav1.2 and Cx40 mRNA and increased the expression of Cx43 in Ep1 group. Compared with T3 group, the expression of Kv1.5,Kv4.3,Cav1.2 and Cx40 mRNA and protein in T3 Epl group was decreased, and the expression of Cx43 was increased. Conclusion: Eplerenone can partially reverse myocardial electrical remodeling induced by thyroid hormone.
【作者单位】：重庆医科大学附属第二医院心内科;
【分类号】：R54

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