原发性高血压合并心力衰竭患者血浆microRNA表达谱的改变
发布时间：2018-11-29 08:47
【摘要】：目的应用微小RNA(miRNA)基因表达谱芯片技术筛查miRNA在原发性高血压(EH)合并心力衰竭患者血浆中的差异表达,旨在进一步探索EH合并心力衰竭发生发展的分子机制。方法收集50例来自医院心血管内科住院部的EH合并心力衰竭急性发作期患者作为实验组。随机选取50例EH不合并心力衰竭患者做为对照组。测定受试者血脂、血压、体质量指数(BMI)、氨基末端脑钠尿肽前体(NT-proBNP)水平,评估临床症状和超声心动图心功能情况,常规抽提总RNA,应用CapitalBio公司miRNA芯片检测miRNA表达谱,并对差异表达的miRNA进行实时荧光定量聚合酶链反应(qRT-PCR)验证,观察相应差异表达的miRNA与NT-proBNP的相关性。结果与对照组比较,实验组血脂、血压、BMI差异无统计学意义(均P0.05)。实验组患者血浆miRNA表达谱中明显差异表达的miRNA共有19个,其中有12个miRNA上调(miR-142-3p、miR-196、miR-28、miR-208等),7个miRNA下调(miR-542、miR-199a、miR-21、miR-195等),与qRT-PCR结果基本相符。miR-142-3p、miR-196与NT-proBNP呈明显正相关。与对照组比较,心功能Ⅱ级患者血浆miR-142-3p和miR-196表达水平分别升高(4.44±0.21)倍和(3.78±0.20)倍(均P0.05),在心功能Ⅲ级患者分别升高(10.09±0.19)倍和(9.88±0.25)倍(均P0.05),在心功能Ⅳ级患者分别升高(15.34±0.18)倍和(13.83±0.22)倍(均P0.05);miR-542、miR-199a与NT-proBNP呈明显负相关。与对照组比较,心功能Ⅱ级患者血浆miR-542和miR-199a表达水平分别降低(91±11)%和(85±10)%(均P0.05),在心功能Ⅲ级患者分别降低(55±9)%和(51±15)%(均P0.05),在心功能Ⅳ级患者分别降低(24±8)%和(22±7)%(均P0.05)。结论 EH合并心力衰竭患者血浆中miRNA的表达有明显改变,并随着心力衰竭严重程度的增加而相应改变,与NT-proBNP明显相关,提示部分血浆miRNA可作为预测EH合并心力衰竭患者疾病进展的新型生物学标志物。
[Abstract]:Objective to screen the differential expression of miRNA in plasma of patients with essential hypertension (EH) complicated with heart failure by microarray RNA (miRNA) gene expression microarray, in order to further explore the molecular mechanism of occurrence and development of EH with heart failure. Methods 50 patients with EH complicated with acute attack of heart failure were selected as experimental group. Fifty patients with EH without heart failure were randomly selected as control group. Serum lipids, blood pressure, body mass index (BMI),) levels of brain natriuretic peptide precursors (NT-proBNP) were measured. Clinical symptoms and echocardiographic cardiac function were evaluated. Total RNA, was extracted by routine extraction. CapitalBio miRNA chip was used to detect the miRNA expression profile, and real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to verify the differentially expressed miRNA. The correlation between the differentially expressed miRNA and NT-proBNP was observed. Results there was no significant difference in blood lipid, blood pressure and BMI between the experimental group and the control group (P 0.05). In the experimental group, 19 miRNA were significantly differentially expressed in the plasma miRNA expression profile, of which 12 miRNA were up-regulated (miR-142-3p,miR-196,miR-28,miR-208 et al) and 7 miRNA were down-regulated (miR-542,miR-199a,miR-21,). MiR-195 et al., which were consistent with the results of qRT-PCR. MiR-142-3p,miR-196 was positively correlated with NT-proBNP. Compared with the control group, the expression of miR-142-3p and miR-196 in patients with cardiac function grade 鈪，
本文编号：2364541
[Abstract]:Objective to screen the differential expression of miRNA in plasma of patients with essential hypertension (EH) complicated with heart failure by microarray RNA (miRNA) gene expression microarray, in order to further explore the molecular mechanism of occurrence and development of EH with heart failure. Methods 50 patients with EH complicated with acute attack of heart failure were selected as experimental group. Fifty patients with EH without heart failure were randomly selected as control group. Serum lipids, blood pressure, body mass index (BMI),) levels of brain natriuretic peptide precursors (NT-proBNP) were measured. Clinical symptoms and echocardiographic cardiac function were evaluated. Total RNA, was extracted by routine extraction. CapitalBio miRNA chip was used to detect the miRNA expression profile, and real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to verify the differentially expressed miRNA. The correlation between the differentially expressed miRNA and NT-proBNP was observed. Results there was no significant difference in blood lipid, blood pressure and BMI between the experimental group and the control group (P 0.05). In the experimental group, 19 miRNA were significantly differentially expressed in the plasma miRNA expression profile, of which 12 miRNA were up-regulated (miR-142-3p,miR-196,miR-28,miR-208 et al) and 7 miRNA were down-regulated (miR-542,miR-199a,miR-21,). MiR-195 et al., which were consistent with the results of qRT-PCR. MiR-142-3p,miR-196 was positively correlated with NT-proBNP. Compared with the control group, the expression of miR-142-3p and miR-196 in patients with cardiac function grade 鈪，
本文编号：2364541
本文链接：https://www.wllwen.com/yixuelunwen/xxg/2364541.html
最近更新
教材专著
