N-WASP在肺炎衣原体感染促进血管新生中的作用

发布时间：2018-12-18 12:00

【摘要】：目的探讨N-WASP在肺炎衣原体感染诱导血管新生中的作用及其可能机制。方法肺炎衣原体增殖培养后感染人血管内皮细胞(VEC),免疫荧光染色确认感染成功。Western blot检测肺炎衣原体感染的VEC内N-WASP磷酸化水平;CCK-8检测N-WASP特异性抑制剂Wiskostain对VEC活力的影响;免疫荧光实验检测工作浓度的Wiskostain对肺炎衣原体感染率的影响;肺炎衣原体感染以5μmol/L Wiskostain预处理的VEC后,管腔形成实验观察各组VEC形成新生血管能力的变化。结果在荧光显微镜下,感染的VEC胞浆内可见典型的肺炎衣原体包涵体。肺炎衣原体感染VEC 10、24 h后N-WASP磷酸化水平均明显上调且高于正常对照组(P0.05)。管腔形成实验结果显示,肺炎衣原体感染VEC 16 h后,其所形成的微管腔节点数明显多于正常对照组(P0.05);经Wiskostain预处理后,肺炎衣原体感染促进微管腔节点形成的作用被显著削弱,几乎不能形成微管腔结构(P0.05)。结论肺炎衣原体感染可能通过N-WASP促进人VEC形成新生血管。
[Abstract]:Objective to investigate the role of N-WASP in angiogenesis induced by chlamydia pneumoniae infection and its possible mechanism. Methods chlamydia pneumoniae proliferation culture infected human vascular endothelial cell (VEC), immunofluorescence staining confirmed successful. Western blot detection of chlamydia pneumoniae infection in VEC phosphorylation level; The effect of Wiskostain, a specific inhibitor of N-WASP, on the activity of VEC was detected by CCK-8, and the infection rate of chlamydia pneumoniae was detected by immunofluorescence assay. Chlamydia pneumoniae infection was pretreated with 5 渭 mol/L Wiskostain VEC, and the angiogenesis ability of VEC in each group was observed by lumen formation experiment. Results typical chlamydia pneumoniae inclusion bodies were found in cytoplasm of infected VEC under fluorescence microscope. The levels of N-WASP phosphorylation in chlamydia pneumoniae were significantly up-regulated 24 h after VEC infection and were significantly higher than those in normal control group (P0.05). The results of lumen formation test showed that the number of microtubule nodes formed by chlamydia pneumoniae infection 16 h after VEC infection was significantly higher than that in normal control group (P0.05). After pretreatment with Wiskostain, the role of Chlamydia pneumoniae infection in promoting the formation of nodes in microtubules was significantly weakened, and almost could not form the structure of microtubules (P0.05). Conclusion Chlamydia pneumoniae infection may promote the formation of neovascularization of human VEC through N-WASP.
【作者单位】：天津医科大学基础医学院生理学与病理生理学系;
【基金】：国家自然科学基金资助项目(81470551;81300206) 高等学校博士点基金项目(20111202110011)
【分类号】：R543.5

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