原发免疫性血小板减少症外周血中T细胞miRNA181a与Treg细胞相关性的分析
发布时间：2018-12-26 17:41
【摘要】：目的:原发免疫性血小板减少症(primary immune thrombocytopenia,ITP),亦称特发性血小板减少性紫癜,是临床上最常见的出血性疾病,临床上以轻到重度血小板减少,相对表现正常的骨髓像和皮肤、粘膜出血为主要临床特征,严重者可因内脏或颅内出血而危及生命。国内外文献表明,ITP患者体内存在T细胞亚群失衡,但触发ITP中T细胞亚群失衡的发病机制并不清楚,可明确地是Treg细胞参与ITP发病。我们通过检测ITP患者及正常人体内T淋巴细胞mi RNA181a表达水平、Treg细胞及其分泌细胞因子TGF-β水平,来探讨mi RNA181a与Treg细胞相关性的分析及两者在ITP发病中所起到的作用。方法:1、外周血T淋巴细胞分离2、通过定量PCR对ITP患者、健康对照组外周血T淋巴细胞mi RNA181a表达水平测定。3、通过流式细胞仪检测ITP患者和健康对照组外周血Treg细胞(CD4+Foxp3+)水平。4、应用ELISA试剂盒检测ITP患者和健康对照组血浆中TGF-β的水平。5、应用SPSS统计学分析软件对所得数据进行统计学处理,并分析T细胞mi RNA181a表达与调节T细胞及其分泌细胞因子相关性结果:1.外周血中T淋巴细胞中m RNA181a的表达量:ITP组:0.495±0.109正常人组:1.296±0.450.ITP组表达量明显低于正常组,P0.001差异有统计学意义。2.外周血中CD4+T淋巴细胞中Treg细胞(CD4+Foxp3)比例(%):ITP组:1.82±0.46。正常人组:7.21±1.08。ITP组比例明显低于正常组,P0.05,差异有统计学意义。3.外周血中TGF-β水平:ITP组:3040.1±265.6正常人组:3442.0±276.6。ITP组比例明显低于正常组,P0.05,差异有统计学意义。4.micro RNA181a与treg细胞相关性分析:micro RNA181a表达水平与treg细胞比例呈正相关,P0.05,r=0.99。结论:1、ITP患者外周血T淋巴细胞mi RNA181a的表达水平下降。2、ITP患者外周血Treg细胞比例及其分泌细胞因子TGF-β降低,说明ITP患者Treg细胞免疫功能受抑。3、ITP患者外周血mi RNA181a表达量与Treg细胞比例呈正相关。4、mi RNA181a表达可能与ITP中发病机制有关。
[Abstract]:Objective: primary immune thrombocytopenia (primary immune thrombocytopenia,ITP), also known as idiopathic thrombocytopenic purpura, is the most common hemorrhagic disease in clinic, with mild to severe thrombocytopenia. Compared with normal bone marrow images and skin, mucosal hemorrhage is the main clinical feature, and severe cases may be life-threatening because of visceral or intracranial hemorrhage. The literature at home and abroad shows that there is an imbalance of T cell subsets in patients with ITP, but the pathogenesis that triggers the imbalance of T cell subsets in ITP is not clear. It is clear that Treg cells are involved in the pathogenesis of ITP. In order to explore the relationship between mi RNA181a and Treg cells and the role of mi RNA181a and Treg cells in the pathogenesis of ITP, we examined the expression of mi RNA181a in T lymphocytes, Treg cells and their secreting cytokines TGF- 尾 in patients with ITP and in normal subjects. Methods: 1. The mi RNA181a expression of T lymphocytes in peripheral blood of ITP patients and healthy controls was measured by quantitative PCR. The levels of Treg cells (CD4 Foxp3) in peripheral blood of ITP patients and healthy controls were detected by flow cytometry, and the levels of TGF- 尾 in plasma of ITP patients and healthy controls were detected by ELISA kit. The data were statistically processed by SPSS software, and the correlation between T cell mi RNA181a expression and regulation of T cell and its secreting cytokines was analyzed as follows: 1. The expression of m RNA181a in peripheral blood T lymphocytes in ITP group: 0.495 卤0.109 normal persons group: 1.296 卤0.450.ITP group was significantly lower than that in normal group, the difference was statistically significant. 2. The proportion of Treg cells (CD4 Foxp3) in CD4 T lymphocytes in peripheral blood (%): ITP: 1.82 卤0.46). Normal group: 7.21 卤1.08.ITP group was significantly lower than the normal group, P 0.05, the difference was statistically significant. The level of TGF- 尾 in peripheral blood of ITP group: 3040.1 卤265.6 normal persons group: 3442.0 卤276.6.ITP group was significantly lower than that of normal group, P 0.05, P 0.05. The correlation analysis between 4.micro RNA181a and treg cells showed that the expression of micro RNA181a was positively correlated with the proportion of treg cells, and the expression of micro RNA181a was positively correlated with the percentage of treg cells. Conclusion: 1 the expression of mi RNA181a in peripheral blood T lymphocytes in patients with ITP decreased. 2 the percentage of Treg cells in peripheral blood and the secretion of TGF- 尾 in peripheral blood of patients with ITP were decreased, which indicated that the immune function of Treg cells was inhibited in ITP patients. The expression of mi RNA181a in peripheral blood of patients with ITP was positively correlated with the proportion of Treg cells, and the expression of RNA181a might be related to the pathogenesis of ITP.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R558.2
[Abstract]:Objective: primary immune thrombocytopenia (primary immune thrombocytopenia,ITP), also known as idiopathic thrombocytopenic purpura, is the most common hemorrhagic disease in clinic, with mild to severe thrombocytopenia. Compared with normal bone marrow images and skin, mucosal hemorrhage is the main clinical feature, and severe cases may be life-threatening because of visceral or intracranial hemorrhage. The literature at home and abroad shows that there is an imbalance of T cell subsets in patients with ITP, but the pathogenesis that triggers the imbalance of T cell subsets in ITP is not clear. It is clear that Treg cells are involved in the pathogenesis of ITP. In order to explore the relationship between mi RNA181a and Treg cells and the role of mi RNA181a and Treg cells in the pathogenesis of ITP, we examined the expression of mi RNA181a in T lymphocytes, Treg cells and their secreting cytokines TGF- 尾 in patients with ITP and in normal subjects. Methods: 1. The mi RNA181a expression of T lymphocytes in peripheral blood of ITP patients and healthy controls was measured by quantitative PCR. The levels of Treg cells (CD4 Foxp3) in peripheral blood of ITP patients and healthy controls were detected by flow cytometry, and the levels of TGF- 尾 in plasma of ITP patients and healthy controls were detected by ELISA kit. The data were statistically processed by SPSS software, and the correlation between T cell mi RNA181a expression and regulation of T cell and its secreting cytokines was analyzed as follows: 1. The expression of m RNA181a in peripheral blood T lymphocytes in ITP group: 0.495 卤0.109 normal persons group: 1.296 卤0.450.ITP group was significantly lower than that in normal group, the difference was statistically significant. 2. The proportion of Treg cells (CD4 Foxp3) in CD4 T lymphocytes in peripheral blood (%): ITP: 1.82 卤0.46). Normal group: 7.21 卤1.08.ITP group was significantly lower than the normal group, P 0.05, the difference was statistically significant. The level of TGF- 尾 in peripheral blood of ITP group: 3040.1 卤265.6 normal persons group: 3442.0 卤276.6.ITP group was significantly lower than that of normal group, P 0.05, P 0.05. The correlation analysis between 4.micro RNA181a and treg cells showed that the expression of micro RNA181a was positively correlated with the proportion of treg cells, and the expression of micro RNA181a was positively correlated with the percentage of treg cells. Conclusion: 1 the expression of mi RNA181a in peripheral blood T lymphocytes in patients with ITP decreased. 2 the percentage of Treg cells in peripheral blood and the secretion of TGF- 尾 in peripheral blood of patients with ITP were decreased, which indicated that the immune function of Treg cells was inhibited in ITP patients. The expression of mi RNA181a in peripheral blood of patients with ITP was positively correlated with the proportion of Treg cells, and the expression of RNA181a might be related to the pathogenesis of ITP.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R558.2
【参考文献】
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1 李慧媛;张冬雷;张岘;付荣凤;宣e，
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