心脉隆对肺动脉平滑肌细胞缺氧损伤的保护作用

发布时间：2019-01-05 11:54

【摘要】：目的:研究心脉隆(XML)注射液对人源性肺动脉平滑肌细胞(PASMCs)缺氧损伤的保护作用,探讨XML降低肺动脉高压(PAH)的可能机制。方法:利用Na2S2O4建立缺氧模拟肺动脉高压细胞缺氧模型,流式细胞术观察缺氧刺激下细胞凋亡率,实时定量PCR检测凋亡相关基因表达,采用激光共聚焦法检测细胞膜电位、胞浆钙离子的表达变化。结果:在缺氧情况下,与对照组比较,PASMCs增殖显著,Bcl-2基因表达增高,Bax表达下调,具有显著性差异(P0.05)。而XML能显著抑制缺氧过程中Bcl-2基因表达上调及Bax基因表达的下调,差异具有统计学意义(P0.05)。此外,XML能稳定细胞膜电位和降低胞浆钙浓度,抑制缺氧诱导的PASMCs增殖。结论:XML可减轻因缺氧引发的PASMCs凋亡减少而导致的平滑肌增殖重塑,为XML用于临床肺动脉高压的治疗提供实验依据。
[Abstract]:Aim: to study the protective effect of Xinmailong (XML) injection on (PASMCs) hypoxia injury in human pulmonary artery smooth muscle cells (PASMC) and to explore the possible mechanism of XML decreasing (PAH) in pulmonary hypertension. Methods: hypoxia simulated pulmonary hypertension cell model was established by Na2S2O4. Apoptosis rate was observed by flow cytometry, apoptosis-related gene expression was detected by real-time quantitative PCR, and cell membrane potential was detected by laser confocal method. Changes in the expression of Ca ~ (2 +) in cytoplasm. Results: compared with the control group, the proliferation of PASMCs was significant, the expression of Bcl-2 gene was increased, and the expression of Bax was down-regulated (P0.05). However, XML could significantly inhibit the up-regulation of Bcl-2 gene expression and the down-regulation of Bax gene expression during hypoxia (P0.05). In addition, XML could stabilize cell membrane potential and decrease cytosolic calcium concentration, and inhibit PASMCs proliferation induced by hypoxia. Conclusion: XML can reduce the proliferation and remodeling of smooth muscle caused by the decrease of PASMCs apoptosis induced by hypoxia, and provide experimental evidence for the treatment of pulmonary hypertension with XML.
【作者单位】：南京医科大学第一附属医院急诊科;宜兴市人民医院ICU;南京医科大学公共卫生学院卫生部毒理实验室;
【基金】：国家自然科学基金(81273115,81473012) 江苏省高校优势学科建设工程资助项目江苏省中医局项目(LZ11081)
【分类号】：R544.1

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