房颤患者射频消融术前后血浆miR-150表达水平的变化

发布时间：2019-01-11 11:38

【摘要】：目的探讨射频消融术前后血浆miR-150表达水平的变化。方法检测60例不同类型房颤患者(房颤组,其中阵发性、持续性和永久性房颤患者分别为26、19和15例)左心房内径、左室射血分数、脑钠尿肽(BNP)、超敏C-反应蛋白(hs-CRP)、D-二聚体水平,不同类型房颤各观察指标的差异;应用实时荧光定量PCR技术检测20例健康体检者(对照组)以及不同类型房颤患者射频消融术前后血浆miR-150表达水平。结果术前不同类型房颤患者左心房内径、左室射血分数、BNP、hs-CRP、D-二聚体水平差异有统计学意义(P均0.05)。对照组血浆miR-150相对表达量为1.00±0.00,房颤组射频消融术前血浆miR-150相对表达量为0.35±0.06,与对照组比较,房颤组术前血浆miR-150相对表达量下降(P0.05)。术前阵发性房颤、持续性房颤和永久性房颤血浆miR-150相对表达量分别为0.53±0.08、0.24±0.07和0.19±0.04,术后相对表达量分别为1.53±0.14、1.67±0.21和0.51±0.13,与术前比较,相同类型房颤射频消融术后血浆miR-150相对表达量均表达上升(P均0.05)。结论血浆miR-150可能参与了房颤发病机制的调控并与房颤病情发展密切相关,可作为房颤诊断、治疗和预后判断的辅助指标。
[Abstract]:Objective to investigate the changes of plasma miR-150 expression before and after radiofrequency ablation. Methods left atrial diameter, left ventricular ejection fraction (LVEF) and brain natriuretic peptide (BNP),) were measured in 60 patients with different types of AF (atrial fibrillation group, 2619 patients with paroxysmal atrial fibrillation and 15 patients with permanent atrial fibrillation). The difference of hs-CRP, D- dimer level and the observed indexes of different types of atrial fibrillation; The expression of plasma miR-150 in 20 healthy subjects (control group) and patients with different types of AF before and after radiofrequency ablation was detected by real-time fluorescence quantitative PCR. Results there were significant differences in left atrial diameter, left ventricular ejection fraction (LVEF) and BNP,hs-CRP,D- dimer level in patients with different types of atrial fibrillation before operation (all P 0. 05). The relative expression of plasma miR-150 was 1.00 卤0.00in the control group and 0.35 卤0.06 in the atrial fibrillation group before radiofrequency ablation. Compared with the control group, the relative expression of plasma miR-150 in the AF group decreased before operation (P0.05). The relative expression of plasma miR-150 in patients with paroxysmal atrial fibrillation, persistent atrial fibrillation and permanent atrial fibrillation were 0.53 卤0.08 卤0.07 and 0.19 卤0.04 before operation, and 1.53 卤0.14 卤0.67 卤0.21 and 0.51 卤0.13 after operation, respectively. Compared with pre-operation, the relative expression of plasma miR-150 increased after radiofrequency ablation of the same type of atrial fibrillation (P0. 05). Conclusion Plasma miR-150 may be involved in the regulation of the pathogenesis of atrial fibrillation and is closely related to the development of atrial fibrillation, which can be used as an auxiliary index for diagnosis, treatment and prognosis of atrial fibrillation.
【作者单位】：大连医科大学附属第一医院;
【分类号】：R541.75

【相似文献】