硫酸吲哚酚对人单核细胞源树突状细胞表型及功能的影响

发布时间：2019-01-12 14:19

【摘要】：目的:探讨硫酸吲哚酚(IS)对人单核细胞源树突状细胞(mDCs)的分化、成熟及免疫功能的影响,为进一步探索IS在动脉粥样硬化(AS)免疫炎症反应中的机制提供依据。方法:双密度梯度离心法分离人外周血单核细胞,用rh GM-CSF和rh IL-4体外诱导分化为未成熟mDCs,随机分为PBS组、LPS组(1μg/m L)、IS.1组(30μmol/L)、IS.2组(300μmol/L)、IS.3组(600μmol/L)。流式细胞仪检测各组mDCs表型和吞噬功能变化,ELISA法检测各组mDCs分泌细胞因子IL-12p70的变化,扫描电子显微镜观察细胞形态学变化。结果:不同浓度IS作用于mDCs后,显著上调mDCs表面标志CD80、CD83、CD86、MHC II的表达,降低细胞吞噬功能并促进细胞因子IL-12p70的分泌(P0.05);形态学上看,LPS组和IS.2组细胞呈不规则形,胞体表面有形态不一的突起。比较得出IS为300μmol/L时为最适宜的刺激浓度。结论:硫酸吲哚酚可诱导人单核细胞源树突状细胞表型及功能成熟,这可能是硫酸吲哚酚参与动脉粥样硬化免疫炎症过程的机制之一。
[Abstract]:Aim: to investigate the effects of indole sulfate (IS) on the differentiation, maturation and immune function of human monocyte derived dendritic cells (mDCs) in order to provide a basis for further exploring the mechanism of IS in the immune inflammation of atherosclerotic (AS). Methods: human peripheral blood mononuclear cells were isolated by double density gradient centrifugation. Rh GM-CSF and rh IL-4 were used to differentiate into immature mDCs,. They were randomly divided into PBS group and LPS group (1 渭 g / m L), IS.1 group (30 渭 mol/L). IS.2 group (300 渭 mol/L) and IS.3 group (600 渭 mol/L). The phenotype and phagocytic function of mDCs in each group were detected by flow cytometry, the cytokine IL-12p70 secreted by mDCs was detected by ELISA method, and the morphological changes were observed by scanning electron microscope. Results: after mDCs was treated with different concentrations of IS, the expression of CD80,CD83,CD86,MHC II on mDCs surface was significantly up-regulated, the phagocytic function was decreased and the secretion of cytokine IL-12p70 was promoted (P0.05). Morphologically, the cells in LPS group and IS.2 group were irregular. The optimal stimulation concentration was obtained when IS was 300 渭 mol/L. Conclusion: indole sulfate can induce the phenotypic and functional maturation of human monocyte derived dendritic cells, which may be one of the mechanisms involved in the immune inflammation of atherosclerosis.
【作者单位】：南方医科大学珠江医院心血管内科;
【基金】：广东省省级科技计划项目(编号:2014A020212544)
【分类号】：R54

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