三种饮用水对高血压小鼠血压的影响
发布时间：2019-01-19 13:04
【摘要】：目的探究3种饮用水对高血压小鼠血压的影响及可能机制。方法测定3种饮用水的水质情况:溶解性总固体(TDS)、耗氧量(OC)、p H、氧化还原电位(ORP)、电导率(EC)、溶解氢、钙和镁;采用N'-硝基-L-精氨酸甲酯盐酸盐(L-NAME)连续灌胃法(30天)建立ICR小鼠高血压模型。然后将小鼠随机分为4组:正常对照组、模型对照组、模型自来水组和模型过滤水组,每个月检测小鼠血压,3个月后测定小鼠血清一氧化氮(NO)、一氧化氮合酶(NOS)、总超氧化物歧化酶(T-SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、内皮素(ET)、血管紧张素(Ang)、醛固酮(ALD)、儿茶酚胺(CA)、白细胞介素-6(IL-6)、超敏反应蛋白(hs-CRP)及环磷酸鸟苷(c-GMP)含量;取小鼠心脏、肾脏和胸主动脉组织制作石蜡切片观察其形态。结果过滤水pH、TDS、OC、EC、钙和镁均高于纯水和自来水,ORP呈负值,且溶解氢含量高;模型过滤水组小鼠收缩压与平均血压[(106.24±5.31)和(90.73±4.99)mmHg]低于模型对照组[(119.58±6.08)和(96.44±6.48)mmHg],差异有统计学意义(P0.05);模型过滤水组NO含量[(87.05±39.82)μmol/L]明显高于模型对照组[(45.01±9.62)μmol/L]和模型自来水组[(46.56±30.54)μmol/L],差异有统计学意义(P0.05);模型自来水组和模型过滤水组IL-6[(201.42±36.41)和(173.99±114.96)pg/mL]和Ang含量[(1319.20±111.90)和(1349.38±180.15)ng/L]显著低于模型对照组[(363.14±149.00)pg/mL和(1736.17±242.86)ng/L],差异有统计学意义(P0.05);模型过滤水组T-SOD和GSH-Px活性[(268.37±12.25)和(712.45±30.59)U/mL]显著高于模型对照组[(250.46±15.60)和(678.36±35.80)U/mL],差异均有统计学意义(P0.05)。结论与纯水、自来水相比,过滤水可通过抗氧化、抗炎作用降低高血压小鼠血压。
[Abstract]:Objective to explore the effect of three kinds of drinking water on blood pressure in hypertensive mice and its possible mechanism. Methods three kinds of drinking water quality were determined: dissolved total solid (TDS), oxygen consumption (OC), p H, redox potential (ORP), conductivity (EC), dissolved hydrogen calcium and magnesium; The hypertensive model of ICR mice was established by continuous intragastric perfusion of Na-nitro-L-arginine methyl ester hydrochloride (L-NAME) for 30 days. Then the mice were randomly divided into four groups: normal control group, model tap water group and model filtered water group. Blood pressure was measured every month and serum nitric oxide (NO), nitric oxide synthase (NOS), was measured after 3 months. Total superoxide dismutase (T-SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), endothelin (ET), angiotensin (Ang), aldosterone (ALD), catecholamine (CA), Interleukin-6 (IL-6), hypersensitive protein (hs-CRP) and cyclic guanosine monophosphate (c-GMP); The hearts, kidneys and thoracic aorta of mice were taken to make paraffin sections to observe their morphology. Results pH,TDS,OC,EC, calcium and magnesium in filtered water were higher than those in pure water and tap water, ORP was negative, and dissolved hydrogen content was high. The systolic blood pressure and mean blood pressure in the model filtered water group [(106.24 卤5.31) and (90.73 卤4.99) mmHg] were significantly lower than those in the model control group [(119.58 卤6.08) and (96.44 卤6.48) mmHg] (P0.05). The content of NO in model filtered water group [(87.05 卤39.82) 渭 mol/L] was significantly higher than that in model control group [(45.01 卤9.62) 渭 mol/L] and model tap water group [(46.56 卤30.54) 渭 mol/L] (P0.05). IL-6 [(201.42 卤36.41) and (173.99 卤114.96) pg/mL] and Ang content [(1319.20 卤111.90) and (1349.38 卤180.15) ng/L] in model tap water group and model filtered water group were significantly lower than those in model control group [(201.42 卤36.41) and (173.99 卤114.96) pg/mL] [ (363.14 卤149.00) pg/mL and (1736.17 卤242.86) ng/L, The difference was statistically significant (P0.05). The activities of T-SOD and GSH-Px in model filtered water group [(268.37 卤12.25) and (712.45 卤30.59) U/mL] were significantly higher than those in model control group [(250.46 卤15.60) and (678.36 卤35.80) U/mL]. The difference was statistically significant (P0.05). Conclusion compared with pure water and tap water, filtered water can reduce blood pressure of hypertensive mice by anti-oxidation and anti-inflammatory effect.
【作者单位】： 福建医科大学卫生检验与检疫学系;厦门大学分子影像暨转化医学研究中心;
【基金】：福建省高校产学合作项目(No.2016Y4004)
【分类号】：R544.1
，
本文编号：2411402
[Abstract]:Objective to explore the effect of three kinds of drinking water on blood pressure in hypertensive mice and its possible mechanism. Methods three kinds of drinking water quality were determined: dissolved total solid (TDS), oxygen consumption (OC), p H, redox potential (ORP), conductivity (EC), dissolved hydrogen calcium and magnesium; The hypertensive model of ICR mice was established by continuous intragastric perfusion of Na-nitro-L-arginine methyl ester hydrochloride (L-NAME) for 30 days. Then the mice were randomly divided into four groups: normal control group, model tap water group and model filtered water group. Blood pressure was measured every month and serum nitric oxide (NO), nitric oxide synthase (NOS), was measured after 3 months. Total superoxide dismutase (T-SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), endothelin (ET), angiotensin (Ang), aldosterone (ALD), catecholamine (CA), Interleukin-6 (IL-6), hypersensitive protein (hs-CRP) and cyclic guanosine monophosphate (c-GMP); The hearts, kidneys and thoracic aorta of mice were taken to make paraffin sections to observe their morphology. Results pH,TDS,OC,EC, calcium and magnesium in filtered water were higher than those in pure water and tap water, ORP was negative, and dissolved hydrogen content was high. The systolic blood pressure and mean blood pressure in the model filtered water group [(106.24 卤5.31) and (90.73 卤4.99) mmHg] were significantly lower than those in the model control group [(119.58 卤6.08) and (96.44 卤6.48) mmHg] (P0.05). The content of NO in model filtered water group [(87.05 卤39.82) 渭 mol/L] was significantly higher than that in model control group [(45.01 卤9.62) 渭 mol/L] and model tap water group [(46.56 卤30.54) 渭 mol/L] (P0.05). IL-6 [(201.42 卤36.41) and (173.99 卤114.96) pg/mL] and Ang content [(1319.20 卤111.90) and (1349.38 卤180.15) ng/L] in model tap water group and model filtered water group were significantly lower than those in model control group [(201.42 卤36.41) and (173.99 卤114.96) pg/mL] [ (363.14 卤149.00) pg/mL and (1736.17 卤242.86) ng/L, The difference was statistically significant (P0.05). The activities of T-SOD and GSH-Px in model filtered water group [(268.37 卤12.25) and (712.45 卤30.59) U/mL] were significantly higher than those in model control group [(250.46 卤15.60) and (678.36 卤35.80) U/mL]. The difference was statistically significant (P0.05). Conclusion compared with pure water and tap water, filtered water can reduce blood pressure of hypertensive mice by anti-oxidation and anti-inflammatory effect.
【作者单位】： 福建医科大学卫生检验与检疫学系;厦门大学分子影像暨转化医学研究中心;
【基金】：福建省高校产学合作项目(No.2016Y4004)
【分类号】：R544.1
，
本文编号：2411402
本文链接：https://www.wllwen.com/yixuelunwen/xxg/2411402.html
最近更新
教材专著
