miR-125b在颈动脉粥样硬化患者外周血单个核细胞中的表达及其对血管平滑肌细胞增殖和迁移的影响
发布时间：2019-01-19 14:01
【摘要】：目的:探讨miR-125b在颈动脉粥样硬化(AS)患者外周血单个核细胞中的表达及对血管平滑肌细胞增殖和迁移的影响。方法:选取85例颈AS患者(不稳定性斑块39例,稳定性斑块46例)和45例健康者(对照组),采用实时荧光定量PCR法检测外周血单个核细胞中miR-125b的表达。培养人主动脉血管平滑肌细胞,分别转染miR-125b模拟物、miR-125b抑制物、miR-125b阴性对照,以不作任何处理的细胞为空白对照组,采用实时荧光定量PCR法检测各组细胞中miR-125b的表达,MTT法检测细胞增殖活性,Transwell细胞迁移实验检测细胞迁移能力。结果:与对照组相比,AS患者外周血单个核细胞中miR-125b相对表达量降低,且不稳定性斑块患者低于稳定性斑块患者(P0.05)。miR-125b模拟物组细胞中miR-125b相对表达量显著高于阴性对照组和空白对照组,且miR-125b抑制物组低于阴性对照组和空白对照组(P0.05);miR-125b模拟物组细胞增殖抑制率和细胞迁移数显著低于阴性对照组和空白对照组,而miR-125b抑制物组则高于阴性对照组和空白对照组(P0.05)。结论:miR-125b可能通过抑制血管平滑肌细胞增殖和迁移而发挥保护动脉的作用。
[Abstract]:Aim: to investigate the expression of miR-125b in peripheral blood mononuclear cells (PBMC) of patients with carotid atherosclerosis (AS) and its effect on proliferation and migration of vascular smooth muscle cells (VSMC). Methods: 85 patients with cervical AS (39 with unstable plaque and 46 with stable plaque) and 45 healthy subjects (control group) were selected to detect the expression of miR-125b in peripheral blood mononuclear cells by real-time fluorescence quantitative PCR. Cultured human aortic vascular smooth muscle cells were transfected with miR-125b analogue, miR-125b inhibitor, miR-125b negative control, and the cells without any treatment were used as blank control group. The expression of miR-125b was detected by real-time fluorescence quantitative PCR assay, cell proliferation activity was detected by MTT assay, and cell migration ability was detected by Transwell cell migration assay. Results: compared with the control group, the relative expression of miR-125b in peripheral blood mononuclear cells of AS patients was decreased. The relative expression of miR-125b in miR-125b analog group was significantly higher than that in negative control group and blank control group. MiR-125b inhibitor group was lower than negative control group and blank control group (P0.05). The cell proliferation inhibition rate and cell migration number in miR-125b mimic group were significantly lower than those in negative control group and blank control group, while those in miR-125b inhibitor group were higher than those in negative control group and blank control group (P0.05). Conclusion: miR-125b may protect arteries by inhibiting the proliferation and migration of vascular smooth muscle cells.
【作者单位】： 新疆石河子大学医学院第一附属医院老干部科;新疆石河子大学医学院第一附属医院神经外科;
【基金】：国家自然科学基金资助项目81360185
【分类号】：R543.4
，
本文编号：2411439
[Abstract]:Aim: to investigate the expression of miR-125b in peripheral blood mononuclear cells (PBMC) of patients with carotid atherosclerosis (AS) and its effect on proliferation and migration of vascular smooth muscle cells (VSMC). Methods: 85 patients with cervical AS (39 with unstable plaque and 46 with stable plaque) and 45 healthy subjects (control group) were selected to detect the expression of miR-125b in peripheral blood mononuclear cells by real-time fluorescence quantitative PCR. Cultured human aortic vascular smooth muscle cells were transfected with miR-125b analogue, miR-125b inhibitor, miR-125b negative control, and the cells without any treatment were used as blank control group. The expression of miR-125b was detected by real-time fluorescence quantitative PCR assay, cell proliferation activity was detected by MTT assay, and cell migration ability was detected by Transwell cell migration assay. Results: compared with the control group, the relative expression of miR-125b in peripheral blood mononuclear cells of AS patients was decreased. The relative expression of miR-125b in miR-125b analog group was significantly higher than that in negative control group and blank control group. MiR-125b inhibitor group was lower than negative control group and blank control group (P0.05). The cell proliferation inhibition rate and cell migration number in miR-125b mimic group were significantly lower than those in negative control group and blank control group, while those in miR-125b inhibitor group were higher than those in negative control group and blank control group (P0.05). Conclusion: miR-125b may protect arteries by inhibiting the proliferation and migration of vascular smooth muscle cells.
【作者单位】： 新疆石河子大学医学院第一附属医院老干部科;新疆石河子大学医学院第一附属医院神经外科;
【基金】：国家自然科学基金资助项目81360185
【分类号】：R543.4
，
本文编号：2411439
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