外周血单核细胞亚群及其趋化因子在急性冠脉综合征早期的表达特点

发布时间：2019-02-17 08:28

【摘要】：目的探讨急性冠脉综合征(acute coronary syndrome,ACS)早期单核细胞亚群及其趋化因子即单核细胞趋化蛋白-1(monocyte chemoattractant protein,MCP-1)和不规则趋化因子(fractalkine,FKN)的表达特点,并分析其相关性。方法选取我院2016年9月至12月以胸痛症状入院拟行冠脉造影术(coronary angiography,CAG)的患者。手术当天术前抽取静脉血,采用流式细胞术检测外周血单核细胞(monocyte,Mon)3个亚型的含量及其比例,依据分化抗原-14(cluster differentiation-14,CD-14)和CD16表达分为3个亚型即CD14+CD16-Mon(Mon1)、CD14+CD16+Mon(Mon2)和CD14-CD16+Mon(Mon3);手术当天术前及术后一天抽取静脉血,ELISA检测Mon1的趋化因子MCP-1和Mon3的趋化因子FKN水平,比较不同组MCP-1-Mon1和FKN-Mon3水平变化,并分析其相关性。结果共入选70例患者,结合其临床症状、心肌标志物、心电图、CAG检查结果进行诊断分组:急性心肌梗死(acute myocardium infarction,AMI)组患者30例、不稳定性心绞痛(unstable angina pectoris,UAP)组患者25例、CAG完全正常者(对照组)15例。流式细胞术结果显示AMI组Mon1所占比例高于UAP组和正常对照组(P0.05),Mon3在各组间尚无差异。AMI组患者外周血Mon3/Mon1比值低于对照组(P0.05)。AMI组和UAP组患者FKN、MCP-1和红细胞分布宽度均高于对照组,并且FKN和Mon3具有强相关性(P0.05;R=0.650 2)。结论单核细胞亚群(Mon1和Mon3)在ACS早期水平增高,并伴有其负责招募的趋化因子(MCP-1和FKN)增加,且FKN和Mon3具有强相关性,提示MCP-1-Mon1和FKN-Mon3两条通路可能参与患者ACS早期病理生理过程。
[Abstract]:Objective to investigate the expression of monocyte subsets and their chemokines (monocyte chemotactic protein-1 (monocyte chemoattractant protein,MCP-1) and irregular chemokine (fractalkine,FKN) in patients with acute coronary syndrome (acute coronary syndrome,ACS). And analyze its correlation. Methods Coronary angiography (coronary angiography,CAG) was performed in our hospital from September to December 2016 with chest pain. On the day of operation, venous blood was drawn before operation, and the contents and proportions of three subtypes of peripheral blood monocyte (monocyte,Mon) were detected by flow cytometry. According to the differentiation antigen-14 (cluster differentiation-14,), the contents of the three subtypes were determined by flow cytometry. CD-14) and CD16 were divided into three subtypes: CD14 CD16-Mon (Mon1), CD14 CD16 Mon (Mon2) and CD14-CD16 Mon (Mon3). Venous blood was taken before operation and one day after operation. The levels of chemokine MCP-1 of Mon1 and chemokine FKN of Mon3 were detected by ELISA. The changes of MCP-1-Mon1 and FKN-Mon3 in different groups were compared and the correlation was analyzed. Results A total of 70 patients were enrolled in this study. 30 patients with acute myocardial infarction (acute myocardium infarction,AMI) and 30 patients with unstable angina pectoris (unstable angina pectoris,) were divided into three groups according to their clinical symptoms, myocardial markers, electrocardiogram and CAG examination results. There were 25 cases in UAP group and 15 cases in normal CAG group (control group). Flow cytometry showed that the proportion of Mon1 in AMI group was higher than that in UAP group and normal control group (P0.05), but there was no difference in Mon3 between each group. The ratio of Mon3/Mon1 in peripheral blood in AMI group was lower than that in control group (P0.05) in). AMI group and UAP group (P0.05). The distribution width of MCP-1 and erythrocyte were higher than that of control group, and FKN and Mon3 had strong correlation (P 0.05). Ru 0.652). Conclusion monocyte subsets (Mon1 and Mon3) increased at the early stage of ACS, accompanied by an increase in the chemokines (MCP-1 and FKN) responsible for recruitment, and FKN and Mon3 were strongly correlated. The results suggest that MCP-1-Mon1 and FKN-Mon3 may be involved in the early pathophysiological process of ACS.
【作者单位】：复旦大学附属华山医院心内科;
【基金】：上海市卫计委中医药科研基金(2014JZ006A) 上海市科委科研计划项目中医类引导项目(15401932000) 国家自然科学基金面上项目(81673701,81573711)~~
【分类号】：R541.4

【相似文献】