生理性缺血训练对动脉粥样硬化进程中兔血管内皮的影响

发布时间：2019-02-27 08:27

【摘要】：目的:研究动脉粥样硬化早期进行生理性缺血训练对斑块形成的影响。方法:成年新西兰白兔24只,随机分为3组,每组8只,分别为对照组、高脂组和训练组。对照组采用普通饮食,其余两组采用高脂饮食,其中训练组左下肢用止血带结扎的方式进行生理性缺血训练3min/次,3次/d,5d/周,另外两组安静笼养。实验周期为4周。结果:4周实验后,兔主动脉油红-O染色结果显示,高脂组主动脉血管壁上动脉粥样硬化斑块面积占1.84±0.83%,而对照组和训练组却没有动脉粥样硬化斑块形成。血管横切面HE染色显示高脂组兔主动脉内膜上已经形成泡沫细胞,而对照组和训练组兔的主动脉内膜光滑,无泡沫细胞形成。实验前三组兔血液VEGF含量组间差异无显著性意义(对照组:8.35±0.37pg/ml,高脂组:8.27±0.55pg/ml,训练组:8.63±0.36pg/ml,P0.05),实验后三组兔血液VEGF含量均高于实验前,但组间差异无显著性意义(对照组:16.93±0.89pg/ml,高脂组:14.39±0.97pg/ml,训练组:14.36±0.73pg/ml,P0.05)。实验前三组兔血液NO含量组间差异无显著性意义(对照组:0.888±0.23μmol/L,高脂组:0.421±0.09μmol/L,训练组:0.529±0.134μmol/L,P0.05),实验后三组兔血液NO含量均高于实验前,组间差异具有显著性意义(P0.01),训练组(2.18±0.144μmol/L)和高脂组(1.82±0.078μmol/L)均显著高于对照组(1.27±0.167μmol/L)。实验前三组兔血液EPCs含量组间差异无显著性意义(对照组:7.25±0.86个/每10~5个淋巴细胞,高脂组:7.5±0.6个/每10~5个淋巴细胞,训练组:8.87±0.61个/每10~5个淋巴细胞,P0.05),实验后训练组EPCs含量(12.75±0.94个/每10~5个淋巴细胞)高于高脂组(8.25±0.73个/每10~5个淋巴细胞)和对照组(8.25±0.73个/每10~5个淋巴细胞)且组间差异具有显著性意义(P0.01)。结论:在动脉粥样硬化形成过程中,生理性缺血训练可以促进机体产生血管内皮相关因子,保护血管内皮功能,减缓粥样硬化斑块形成速度。
[Abstract]:Objective: to study the effect of physiological ischemia training on plaque formation in early stage of atherosclerosis. Methods: twenty-four adult New Zealand white rabbits were randomly divided into three groups: control group, hyperlipidemia group and training group. Normal diet was used in the control group and high fat diet was used in the other two groups. In the training group, the left lower limbs were treated with tourniquet ligation for physiological ischemia training for 3min/ times, 3 times a day, 5 days a week, and the other two groups were quietly caged. The experiment period was 4 weeks. Results: after 4 weeks of experiment, the oil red-O staining of rabbit aorta showed that the area of atherosclerotic plaque was 1.84 卤0.83% in the hyperlipidemic group, but there was no atherosclerotic plaque in the control group and training group. The cross-sectional HE staining showed that foam cells had been formed in the aortic intima of the hyperlipidemia group, while the aortic intima of the control group and the training group were smooth and no foam cells were formed. There was no significant difference in blood VEGF content among the three groups before the experiment (control group: 8.35 卤0.37 PG / ml, high fat group: 8.27 卤0.55 PG / ml, training group: 8.63 卤0.36 PG / ml,P0.05). After the experiment, the blood VEGF levels of the three groups were all higher than those before the experiment, but there was no significant difference between the three groups (control group: 16.93 卤0.89 PG / ml, high fat group: 14.39 卤0.97pg / ml, training group: 14.36 卤0.73 PG / ml,P0.05). There was no significant difference among the three groups before the experiment (control group: 0.888 卤0.23 渭 mol / L, high fat group: 0.421 卤0.09 渭 mol / L, training group: 0.529 卤0.134 渭 mol / L, P0.05). After the experiment, the blood NO contents of the three groups were all higher than those before the experiment, and there was a significant difference between the three groups (P0.01). The training group (2.18 卤0.144 渭 mol / L) and the hyperlipidemia group (1.82 卤0.078 渭 mol / L) were significantly higher than the control group (1.27 卤0.167 渭 mol / L). There was no significant difference in blood EPCs content among the three groups before the experiment (control group: 7.25 卤0.86 / 10 / 5 lymphocytes, hyperlipidemic group: 7.5 卤0.6 / 10 / 5 lymphocytes, control group: 7.25 卤0.86 / 10 / 5). Training group: 8.87 卤0.61 lymphocytes / 5 lymphocytes per 10 days, P0.05). After the experiment, the EPCs content in the training group (12.75 卤0.94 / 10 / 5 lymphocytes) was higher than that in the hyperlipidemia group (8.25 卤0.73 / 10 / 5 lymphocytes) and the control group (8.25 卤0.73 / 10 / 5 lymphocytes). The difference was significant (P0.01). Conclusion: in the process of atherosclerosis, physiological ischemia training can promote the production of vascular endothelial factors, protect vascular endothelial function, and slow down the formation of atherosclerotic plaques.
【作者单位】：南京林业大学体育部;南京医科大学第一附属医院康复医学科;南京医科大学附属南京医院(南京市第一医院)心内科;南京体育学院运动健康科学系;南京医科大学附属南京医院(南京市第一医院)康复医学科;
【基金】：国家自然科学基金项目(81101456) 南京市医学科技发展资金项目(QRX11248,YKK14097) 江苏省高校自然科学基金项目(14KJB320002) 南京医科大学科技发展基金项目(2013NJMU077)
【分类号】：R543.5

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