外周血中GBGT1基因高表达可能作为评估急性心肌梗死风险的分子标记

发布时间：2021-05-27 12:30

　　目的:研究外周血中GBGT1基因高表达是否可能作为急性心肌梗死风险评估的遗传标记,并讨论GBGT1基因参与急性心肌梗死发病的可能机制。方法:在中国东北汉族人群中,选取急性心肌梗死病人90例为病例组,冠脉正常者90例为对照组,两组病人均经冠脉造影明确诊断。详细记录每位研究对象的临床资料。采用实时荧光定量PCR检测GBGT1基因在外周血中mRNA水平的表达。结果:在中国东北汉族人群中,急性心梗病人外周血白细胞中GBGT1基因mRNA表达增高;两组间血糖、血脂等生化指标比较显示:心梗组空腹血糖高于对照组,高密度脂蛋白低于对照组。结论:GBGT1基因在急性心肌梗死病人外周血白细胞中表达显著增高;外周血中GBGT1的高表达可能作为急性心肌梗死风险评估的分子标记。 

【文章来源】：吉林大学吉林省 211工程院校 985工程院校 教育部直属院校

【文章页数】：47 页

【学位级别】：硕士

【文章目录】：
中文摘要
ABSTRACT
chapter 1 introduction
Chapter 2 literature review
    2.1 Mechanism of myocardial infarction
    2.2 Atherosclerosis formation process
    2.3 The familial risk of myocardial infarction
    2.4 Symptoms and signs of myocardial infarction
    2.5 Myocardial infarction diagnosis
        2.5.1 The electrocardiogram(ECG)
    2.6 Management of acute myocardial infarction
        2.6.1 Pre-hospital management
        2.6.2 Management initiated in hospital
        2.6.3 Primary PCI
        2.6.4 Fibrinolytic drugs
        2.6.5 Thrombolytic therapy
        2.6.6 Coronary bypass surgery
        2.6.7 Other initial managements
Chapter 3 research objectives and methods
    3.1 Research objectives
    3.2 Exclusion criteria for this study:
    3.3 Research methods
        3.3.1 Peripheral blood collection
        3.3.2 Total RNA extraction and cDNA synthesis from peripheralblood
        3.3.3 Real-time quantitative PCR detection and identification ofamplified products
    3.4 Statistical analysis
    3.5 Ethics Statement
Chapter 4. Results
    4.1 Analysis of clinical data of study subjects
    4.2 Real-time quantitative PCR detection results analysis
    4.3 Analysis of the relationship between the relative expression levelof GBGT1 gene and troponin in the myocardial infarction group
    4.4 Analysis of the relationship between the relative expression ofGBGT1 gene and age
    4.5 Analysis of the relationship between the relative expression ofGBGT1 gene and blood type
    4.6 Logistic regression analysis of the relationship between therelative expression of GBGT1 gene and other factors and theincidence of acute myocardial infarction
Chapter 5. Discussion
Chapter 6. Conclusion
References
Author’s Introduction
Acknowledgement


【参考文献】：
期刊论文
[1]Recent advances in the diagnosis and treatment of acute myocardial infarction[J]. Koushik Reddy,Asma Khaliq,Robert J Henning.  World Journal of Cardiology. 2015(05)



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