注射用前列地尔及人参皂苷Rg3对大鼠心肌缺血/再灌注损伤的影响及机制研究

发布时间：2022-01-08 02:23

　　第一部分注射用前列地尔对大鼠心肌缺血/再灌注损伤的影响目的:观察注射用前列地尔对大鼠心肌缺血/再灌注损伤的影响,并初步探讨其作用机制。方法:（1）选用健康雄性SD大鼠,随机分为4组:假手术组（Sham组）,心肌缺血/再灌注模型组（I/R组）,前列地尔低剂量组（Alprostadil 4μg/kg）,前列地尔高剂量组（Alprostadil 8μg/kg）。（2）结扎大鼠左冠状动脉前降支使其缺血30min,再灌注24h,制备心肌缺血/再灌注损伤模型。再灌注24h后检测血清肌钙蛋白T（Troponin T）、肌酸激酶同工酶（CK-MB）及乳酸脱氢酶（LDH）水平、NBT法检测心肌梗死面积、应用超声心动图检测大鼠心功能（再灌注72h）、在光镜及电镜下观察心肌组织形态学特点、TUNEL原位标记法和DAPI双重染色检测大鼠心肌细胞凋亡、检测心肌组织丙二醛（MDA）含量、超氧化物歧化酶（SOD）及过氧化氢酶（CAT）活性。结果:（1）与Sham组相比,模型组血清Troponin T、CK-MB及LDH均增高,差异有统计学意义（p<0.05）。与模型组相比,前列地尔4或8μg/kg组血清Tro... 

【文章来源】：吉林大学吉林省 211工程院校 985工程院校 教育部直属院校

【文章页数】：122 页

【学位级别】：博士

【部分图文】：

4，电镜下观察心肌细胞，模型组大鼠心肌细胞细胞核和线粒体都发生了严重损伤，表现为心肌肌节纤维排列紊乱，肌丝溶解、断裂、Z线不清；

【参考文献】：
期刊论文
[1]Myocardial ischemia is a key factor in the management of stable coronary artery disease[J]. Kohichiro Iwasaki.  World Journal of Cardiology. 2014(04)
[2]Cardioprotective effect of liposomal prostaglandin E1 on a porcine model of myocardial infarction reperfusion no-reflow[J]. Jia-hui LI,Peng YANG,Ai-li LI,Yong WANG,Yuan-nan KE,Xian-lun LI (Department of Cardiology,China-Japan Friendship Hospital,Beijing 100029,China).  Journal of Zhejiang University-Science B（Biomedicine & Biotechnology）. 2011(08)
[3]Effects of Extracts from Panax Notoginseng and Panax Ginseng Fruit on Vascular Endothelial Cell Proliferation and Migration in vitro[J]. 雷燕,高倩,陈可冀.  Chinese Journal of Integrative Medicine. 2008(01)
[4]Inhibitory effect of ginsenoside Rb1 on calcineurin signal pathway in cardiomyocyte hypertrophy induced by prostaglandin F2α[J]. Qing-song JIANG~2 Xie-nan HUANG Gui-zhong YANG~4 Xiao-yan JIANG~5 Qi-xin ZHOU~(2,6) ~2Department of Pharmacology,Chongqing Medical University,Chongqing 400016,China;~3Department of Pharmacology,Zunyi Medical College,Zunyi 563003,China;~4Department of Biochemistry Zunyi Medical College,Zunyi 563003,China;~5Chengdu Vocational College of Agricultural Science and Technology,Chengdu 611100,China.  Acta Pharmacologica Sinica. 2007(08)
[5]前列腺素E1在心内直视术中对再灌注心肌的影响[J]. 何正富,郦志军.  浙江大学学报(医学版). 2004(04)
[6]Effect of Gαq/11 protein and ATP-sensitive potassium channels on prostaglandin E1 preconditioning in rat hearts[J]. Shou-guo MA, Run-fang FU1,2, Guo-qing1 FENG, Zhen-ji WANG1, Xiang-qin MA1, Shi-ai WENG1Department of Cardiology, The 159th hospital of PLA, Zhumadian 463000, China;1Department of Pharmacology, Medical College, Zhengzhou University, Zhengzhou, 450052, China.  Acta Pharmacologica Sinica. 2004(05)



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