d-δ-三烯生育酚对人晶状体上皮SRA细胞增殖和凋亡的影响

发布时间：2017-12-27 21:03

本文关键词：d-δ-三烯生育酚对人晶状体上皮SRA细胞增殖和凋亡的影响　出处：《眼科新进展》2015年08期 　论文类型：期刊论文

【摘要】：目的研究d-δ-三烯生育酚对人晶状体上皮SRA细胞增殖和凋亡的影响,探讨后发性白内障治疗的新方法。方法将人晶状体上皮SRA细胞分为对照组和实验组,对照组不加药物干预,实验组设3个药物浓度,分别为30μmol·L-1、40μmol·L-1、50μmol·L-1的d-δ-三烯生育酚,药物干预人晶状体上皮SRA细胞24 h和48 h后,采用MTT法、Hoechst33258荧光染色和流式细胞仪分析人晶状体上皮SRA细胞的增殖和凋亡情况。结果 d-δ-三烯生育酚呈浓度-时间依赖性抑制人晶状体上皮SRA细胞的增殖,诱导其凋亡。30μmol·L-1、40μmol·L-1、50μmol·L-1的d-δ-三烯生育酚干预48 h对SRA细胞的增殖抑制率分别为(32.23±5.25)%、(54.17±1.63)%和(86.80±0.85)%,与对照组比较差异均有统计学意义(均为P0.05);干预24 h增殖抑制率分别为(12.70±1.20)%、(19.53±0.95)%和(51.83±6.11)%,与对照组相比差异均有统计学意义(均为P0.05)。干预24 h后,各实验组细胞晚期凋亡率Q2分别为(5.87±0.15)%、(11.37±0.85)%、(22.77±2.41)%,与对照组的(1.10±0.17)%相比,差异均有统计学意义(均为P0.05);各实验组细胞早期凋亡率Q4分别为(3.57±0.32)%、(4.20±0.40)%、(8.00±0.50)%,与对照组的(2.53±0.15)%相比,差异均有统计学意义(均为P0.05)。结论 d-δ-三烯生育酚抑制人晶状体上皮SRA细胞增殖,诱导其凋亡,具有抗晶状体上皮细胞增殖的生物学作用,为治疗后发性白内障提供一种新思路。
[Abstract]:Objective to study the effect of d- Delta - three - tocopherol on the proliferation and apoptosis of human lens epithelial SRA cells and to explore a new method for the treatment of posterior - onset cataract. Methods human lens epithelial SRA cells were divided into control group and experimental group, control group without drug intervention, the experimental group with 3 concentration were 30 mol L-1 and 40 mol L-1, 50 mol - L-1 d- - three ene delta tocopherol, drug intervention of human lens epithelial SRA cells 24 h and 48 h, using the MTT method, Hoechst33258 staining and analysis of cell proliferation and apoptosis of human lens epithelial SRA cells by flow cytometry. Results d- Delta - three - ene tocopherol inhibited the proliferation of human lens epithelial SRA cells in a concentration - time dependent manner and induced apoptosis. 30 mol L-1, 40 mol, 50 mol - L-1 - L-1 - d- delta three ene 48 h intervention on SRA tocopherol cell proliferation inhibition rates were (32.23 + 5.25)% and (54.17 + 1.63)% and (86.80 + 0.85)%, compared with the control group were significant the difference (P0.05); intervention 24 h proliferation inhibition rate (12.70 + 1.20)% and (19.53 + 0.95)% and (51.83 + 6.11)%, compared with the control group, the differences were statistically significant (P0.05). After treatment for 24 h, the cells of each experimental group late apoptosis rate of Q2 was (5.87 + 0.15)% and (11.37 + 0.85)% and (22.77 + 2.41)%, and the control group (1.10 + 0.17)%, the differences were statistically significant (P0.05); the experimental group of early apoptosis the rate of Q4 were (3.57 + 0.32)% and (4.20 + 0.40)% and (8 + 0.50)%, and the control group (2.53 + 0.15)%, the differences were statistically significant (P0.05). Conclusion d- delta three ene tocopherol inhibits the proliferation and induces apoptosis of human lens epithelial SRA cells, which has a biological effect on the proliferation of lens epithelial cells, and provides a new idea for the treatment of posterior capsule opacification.
【作者单位】：桂林医学院附属医院眼科;桂林医学院科学实验中心;
【分类号】：R779.66
【正文快照】： l l 丁芝祥出生,湖南新化人,眼科副主任医师。联系电话:13978361092;E-mail:dudu200488@126.comAbout ZENG Xin-Sheng:Male,bornin May,1971.Deputy chief physician.Tel:13978361092;E-mail:dudu200488@126.com修回日期:2015-04-03Accepted date:Apr 3,2015From the Departme

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