MicroRNA-98对视网膜母细胞瘤Y79细胞增殖和转移的影响

发布时间：2017-12-27 22:05

本文关键词：MicroRNA-98对视网膜母细胞瘤Y79细胞增殖和转移的影响　出处：《实用医学杂志》2015年24期 　论文类型：期刊论文

【摘要】：目的:探讨microRNA-98(miR-98)对视网膜母细胞瘤Y79细胞增殖和转移的影响。方法:将人视网膜母细胞瘤细胞株Y79分为四组:miR-98干预组、阴性miRNA对照组、空白脂质体组和空白对照组。通过脂质体介导,将miR-98的拟似物(miR-98mimics)转染入上述四组细胞,逆转录聚合酶链式反应(RTPCR)检测细胞miR-98的表达,MTT法检测细胞增殖抑制率,流式细胞术检测细胞周期,Transwell实验检测细胞侵袭能力变化。Western blot技术检测细胞信号分子Ras的表达。结果:脂质体转染成功地将miR-98mimics转染入Y79细胞,干预组miR-98的表达水平(89.15±3.25)显著高于对照组、空白脂质体组和空白对照组,且4组间有显著性差别(F=219.17 P0.001)。miR-98对Y79细胞增殖有明显抑制作用。Y79细胞周期被抑制在G1期。对照组和干预组穿膜细胞数分别为(181.2±4.1)个/高倍视野和(50.6±3.5)个/高倍视野,两组相比有显著性差别(t=39.18,P0.01),miR-98抑制了Y79细胞的侵袭能力。miR-98表达增高的Y79细胞的信号分子Ras表达下降。结论:miR-98可以抑制视网膜母细胞瘤Y79细胞增殖和转移,且负调控原癌基因Ras的表达。
[Abstract]:Objective: To investigate the effect of microRNA-98 (miR-98) on the proliferation and metastasis of Y79 cells in retinoblastoma. Methods: the human retinoblastoma cell line Y79 was divided into four groups: the miR-98 intervention group, the negative miRNA control group, the blank liposome group and the blank control group. Mediated by liposome, the miR-98 analogue (miR-98mimics) was transfected into the four cells, reverse transcriptase polymerase chain reaction (RTPCR) to detect the expression of miR-98 cells, cell proliferation was analyzed by MTT assay, flow cytometry, Transwell test cell invasion ability changes. Western blot technique was used to detect the expression of cell signaling molecule Ras. Results: liposome transfection successfully transfected miR-98mimics into Y79 cells. The expression level of miR-98 in the intervention group (89.15 + 3.25) was significantly higher than that in the control group, blank liposome group and blank control group, and there was a significant difference between the 4 groups (F=219.17 P0.001). MiR-98 has a significant inhibitory effect on the proliferation of Y79 cells. The cell cycle of Y79 is inhibited in the G1 phase. The number of penetrating cells in the control group and intervention group was (181.2 + 4.1) / high-power field and (50.6 + 3.5) / high-power field, respectively. There was a significant difference between the two groups (t=39.18, P0.01), and miR-98 inhibited the invasive ability of Y79 cells. The expression of signal molecule Ras in Y79 cells with increased expression of miR-98 decreased. Conclusion: miR-98 can inhibit the proliferation and metastasis of Y79 cells and negatively regulate the expression of proto oncogene Ras.
【作者单位】：河北医科大学第二医院眼科;新疆医科大学第五附属医院检验科;河北医科大学第二医院肿瘤中心;
【分类号】：R739.7
【正文快照】： 婴幼儿发育期眼内恶性肿瘤之一当属视网膜母细胞瘤(retinoblastoma,RB),对其致病机制的深入研究将有助于RB的早期诊断和靶向治疗。近年来micro RNA与RB发生发展的关系已得到学术界的广泛关注,有报道mi R-98 在正常视网膜组织中高表达而在RB中低表达,但其确切机制还很不清楚[1

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