纳豆激酶对兔缺血型视网膜病变模型影响的实验研究

发布时间：2018-03-22 21:13

本文选题：纳豆激酶　切入点：视网膜中央静脉阻塞　出处：《南京医科大学》2010年硕士论文　论文类型：学位论文

【摘要】：目的:用氩激光光凝视网膜中央静脉制作兔视网膜中央静脉阻塞(CRVO)模型,玻璃体腔注射纳豆激酶评价其对视网膜的作用。方法:采用氩激光直接光凝法造成兔CRVO模型,并通过眼底荧光素钠血管造影(FFA)及眼底照相证实造模成功后将其分为3组,每组12只眼,纳豆激酶A组连续3天玻璃体腔注射0.1 FU纳豆激酶提取液0.1 ml;林格氏液对照B组同方法注射林格氏液0.1 ml;空白模型对照C组未外加处理。3组均在光凝前,光凝后进行眼底照相,FFA检测;光凝前及光凝后2周3组兔进行闪光视网膜电流图(flash electroretinogram ,FERG)检查和眼球壁病理切片苏木精-伊红染色观察。结果:①激光可以建立兔CRVO模型,并模拟了人的CRVO疾病发展进程;造模成功后FFA显示视网膜中央静脉血流中断,末梢血管充盈扩张,②激光后2周,C组兔眼FERG a、b波潜伏期较造模前明显延长,振幅降低;③激光后2周,A组FERG a、FERG b波潜伏期较B、C组明显短,振幅较B、C组明显大(P0.05)④与激光造模前相比,A组兔眼FERG a、FERG b波潜伏期延长,振幅降低;⑤A组与B组相比,病理切片观察显示前者视网膜细胞排列紧密,数量较多,后者有明显网膜结构疏松。结论:氩激光致兔CRVO模型是可行成功的,FERG和病理结果提示纳豆激酶可部分改善视网膜功能。
[Abstract]:Aim: to establish a rabbit model of central retinal vein occlusion (CRVOO) by argon laser photocoagulation, and to evaluate the role of nattokinase by intravitreal injection. Methods: the rabbit CRVO model was established by direct photocoagulation with argon laser. The model was confirmed by fundus fluorescein angiography (FFA) and fundus photography. The model was divided into 3 groups with 12 eyes in each group. In group A, 0.1 FU natto kinase extract was injected into vitreous cavity for 3 consecutive days; Linger's solution was injected with 0.1 ml in group B in the same way; group C in blank model group was treated with untreated 0.1 ml before photocoagulation. The fundus radiography (FFA) was performed before photocoagulation and 2 weeks after photocoagulation, and flash electroretinogram FERG and hematoxylin-eosin staining were performed in 3 groups of rabbits before and 2 weeks after photocoagulation. Results the CRVO model of rabbit could be established by 1: 1 laser, and the development process of human CRVO disease was simulated. After the successful establishment of the model, FFA showed the interruption of blood flow in the central retinal vein. In group C, the latency of FERG a b wave in group C was significantly longer than that in group C, and the latency of wave B in group A was shorter than that in group C 2 weeks after laser filling and dilatation of peripheral blood vessels, and the amplitudes decreased 2 weeks after laser irradiation, and the latency of wave B in group A was significantly shorter than that in group C. The amplitude of group A was significantly longer than that of group B (P 0.05) compared with that of group A (before laser modeling), and the latency of wave B in group A was longer than that of group A, and the amplitude of group A was lower than that of group B. The histopathological observation showed that the retinal cells of group A were arranged closely and the number of retinal cells was more than that of group B. The latter has a loose reticular membrane structure. Conclusion: rabbit CRVO model induced by argon laser is feasible and successful, and the pathological results suggest that nattokinase can partially improve retinal function.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R774.1

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