N-甲基-D-天冬氨酸诱导的正常眼压青光眼小鼠模型的实验研究

发布时间：2018-04-03 21:14

本文选题：青光眼　切入点：动物模型　出处：《武汉大学学报(医学版)》2014年05期

【摘要】：目的:利用N-甲基-D-天冬氨酸(NMDA)诱导的正常眼压青光眼小鼠模型,研究NMDA与视网膜神经节细胞(RGCs)损伤的关系。方法:6周龄C57BL/6J小鼠随机分为低、中、高三个剂量组,玻璃体腔内分别注射NMDA 10,20,30nmol,产生视网膜损伤动物模型。在整体动物水平通过眼底照相、视网膜电图(ERG);在组织学水平通过视网膜HE染色、视神经甲苯胺蓝染色、全视网膜铺片Brn-3a免疫荧光染色及RGCs计数观察术后6h,12h,24h,48h,7dRGCs的损伤情况。结果:NMDA干预后24h,RGCs数量明显减少,内丛状层厚度变薄。眼底视网膜呈苍白色改变,血管迂曲痉挛;ERG显示a波和b波潜伏期的变化不大,b波振幅下降。光镜下可见RGCs密度逐渐降低;视神经轴索呈退行性变,与NMDA的作用时间呈正相关。RGCs计数显示NMDA诱导的RGC减少主要集中于术后1d,24hRGCs数量减少83.97%(P0.01)。结论:玻璃体腔内注射NMDA可在不影响眼压的情况下诱导RGCs的显著减少和功能损害,与急性青光眼性损伤具有相似性,可为视神经保护研究提供研究条件。
[Abstract]:Aim: to study the relationship between NMDA and retinal ganglion cells (RGCs) injury in normal intraocular pressure glaucoma mice induced by N- methyl-Daspartic acid.Methods C57BL/6J mice at 6 weeks of age were randomly divided into three groups: low, middle and high dose groups, and intravitreous injection of NMDA 1010 and 20U 30nmol, respectively, to produce retinal injury animal model.At the whole animal level, ERGCs were taken by fundus radiography, electroretinogram (ERG), retinal HE staining, optic nerve toluidine blue staining, Brn-3a immunofluorescence staining and RGCs count. The injury of RGCs was observed at 6 h, 12 h, 24 h, 48 h and 7 d after operation.Results the number of RGCs decreased and the thickness of the inner plexiform layer became thinner at 24 h after intervention.The retinal changes of the fundus were pale, and the amplitude of B wave and a wave were not decreased by ERG in vasospasm and vasospasm.Under light microscope, the density of RGCs decreased gradually, and the optic axons showed degeneration, which was positively correlated with the action time of NMDA. RGCs count showed that the decrease of RGC induced by NMDA was mainly focused on the reduction of RGCs at 24 hours after 1 day of operation (83.97%, P 0.01).Conclusion: intravitreal injection of NMDA can significantly reduce and damage the function of RGCs without affecting intraocular pressure, which is similar to that of acute glaucoma injury, and may provide the research conditions for the study of optic nerve protection.
【作者单位】：武汉大学人民医院眼科中心;
【基金】：国家自然科学基金面上项目(编号:81270998) 卫生部公益性行业科研专项(编号:201302015) 武汉大学自主科研拔尖创新人才项目(编号:2042014kf0207)
【分类号】：R775;R-332

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