18β-甘草次酸对变应性鼻炎大鼠鼻黏膜上皮细胞紧密连接的影响

发布时间：2018-05-12 00:17

本文选题：β-甘草次酸 + 鼻炎　；参考：《临床耳鼻咽喉头颈外科杂志》2014年20期

【摘要】：目的:观察18β-甘草次酸(GA)对变应性鼻炎(AR)大鼠鼻黏膜上皮细胞紧密连接(TJs)超微结构的影响。方法:96只Wistar大鼠随机分为对照组、模型组、氯雷他定组、GA组,每组24只。卵清蛋白致敏建立大鼠AR模型,观察和比较给药干预后第2、4、6、10周,各组行为改变及透射电镜下鼻黏膜上皮细胞TJs形态。结果:AR模型TJs长度变短,质膜电子高密度影变厚、融合点数量减少,间隙增宽甚至断裂。在变应原持续作用下,模型组鼻黏膜上皮细胞TJs的上述改变呈进行性加重,而GA组TJs变短、质膜影变厚、膜融合点减少均较模型组缓解,并随观察周期延长基本接近对照组。结论:18β-GA可缓解AR大鼠鼻腔黏膜上皮细胞间TJs的损害。
[Abstract]:Aim: to observe the effect of 18 尾 -glycyrrhetinic acid (GAGA) on the ultrastructure of nasal epithelial cells (TJs) in rats with allergic rhinitis. Methods Ninety-six Wistar rats were randomly divided into control group, model group and loratadine group with 24 rats in each group. Rat AR model was established by sensitizing ovalbumin. The behavior of each group and TJs morphology of nasal epithelial cells under transmission electron microscope (TEM) were observed and compared at the 6th week after administration of ovalbumin. Results the length of TJs in the 1: AR model is shorter, the electron density shadow of plasma membrane is thicker, the number of fusion points is reduced, and the gap is widened or even broken. Under the continuous action of allergen, the above changes of TJs in nasal epithelial cells in the model group were progressively aggravated, while in the GA group, the TJs became shorter, the plasma membrane shadow thickened, and the decrease of the membrane fusion point were alleviated compared with the model group, and the changes were similar to those in the control group with the prolongation of the observation period. Conclusion 1: 18 尾-GA can alleviate the damage of TJs in nasal mucosal epithelium of AR rats.
【作者单位】：兰州大学第一医院耳鼻咽喉头颈外科;甘肃中医学院附属医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金项目(No:81160449) 甘肃省卫生厅行业科技计划项目(No:GSWST2011-06) 甘肃省中医药科学技术研究课题(No:GZK-2011-22)
【分类号】：R765.21

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