三七姜挥发油抑制鼻咽癌CNE-2细胞增殖的作用

发布时间：2018-06-01 11:50

本文选题：三七姜 + 挥发油　；参考：《中国医院药学杂志》2014年09期

【摘要】：目的:体外培养法研究三七姜挥发油对鼻咽癌CNE-2细胞株生长的抑制作用和作用机制。方法:运用MTT法、Hoechst33258荧光染色法、流式细胞仪和倒置荧光显微镜进行研究。结果:三七姜挥发油对鼻咽癌CNE-2细胞增殖具有抑制作用,且呈剂量依赖性和时间依赖性。作用24 h后CNE-2细胞的IC50为304.8μg·ml-1;Hoechst33258荧光染色法观察细胞核有明显的凋亡形态变化;FITC-Annexin V/PI双染法发现三七姜挥发油能诱导CNE-2细胞凋亡,且随着剂量的增加作用增强;PI染色法说明三七姜挥发油将CNE-2细胞阻滞于G1期。结论:三七姜挥发油能有效抑制CNE-2细胞的生长,其抗肿瘤机制为诱导肿瘤细胞凋亡。
[Abstract]:Aim: to study the inhibitory effect and mechanism of volatile oil of Panax notoginseng on the growth of nasopharyngeal carcinoma cell line CNE-2 in vitro. Methods: MTT method and Hoechst 33258 fluorescence staining were used, flow cytometry and inverted fluorescence microscope were used to study. Results: the essential oil of Panax notoginseng inhibited the proliferation of nasopharyngeal carcinoma CNE-2 cells in a dose-and time-dependent manner. 24 h after exposure, the IC50 of CNE-2 cells was 304.8 渭 g ml-1H Hoechst33258 fluorescence staining. The apoptosis of CNE-2 cells was observed by fluorescence staining. FITC-Annexin V / Pi double staining showed that the volatile oil of Panax notoginseng could induce apoptosis of CNE-2 cells. The results showed that the essential oil of Panax notoginseng could block CNE-2 cells in G1 phase. Conclusion: the essential oil of Panax notoginseng can effectively inhibit the growth of CNE-2 cells, and its antitumor mechanism is to induce apoptosis of CNE-2 cells.
【作者单位】：桂林医学院;
【基金】：广西科技厅重大专项计划资助(编号:12118005-2-3) 广西教育厅重点资助项目(编号:201202ZD067)
【分类号】：R739.63

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