玻璃体内注射索拉非尼对大鼠氧诱导视网膜病变模型新生血管的作用

发布时间：2018-09-04 17:22

【摘要】：目的探讨玻璃体内注射索拉非尼对氧诱导视网膜病变(oxygen-induced retinopathy,OIR)大鼠模型视网膜新生血管的作用。方法 7 d龄SD乳鼠144只,随机分为6组:A组:对照组;B组:ROP组;C组:ROP溶媒对照组;D组:ROP+5μg索拉非尼组;E组:ROP+20μg索拉非尼组;F组:ROP+80μg索拉非尼组。除A组在正常氧环境下饲养外,其余各组乳鼠均建立OIR模型。乳鼠于生后第12天出氧箱后给予玻璃体内注射DMSO及相应剂量的索拉非尼,于生后第17天处死,做视网膜铺片,并于激光共聚焦显微镜下观察视网膜血管形态并在高倍视野下计数血管分支点数目;组织切片HE染色观察视网膜组织形态并计数突破内界膜的血管内皮细胞核数。结果视网膜铺片显示,B组和C组视网膜血管迂曲扩张并形成大量新生血管,经索拉非尼治疗后,视网膜新生血管大幅度减少,且呈剂量依赖性。A组、B组、C组、D组、E组、F组血管分支点数目分别为(16.50±3.90)个、(37.44±6.47)个、(37.08±5.10)个、(30.80±6.85)个、(26.08±5.08)个、(19.83±3.51)个;除B组与C组差异无统计学意义外,其余各组间差异均有统计学意义(均为P0.05)。HE染色显示,B组和C组有大量突破内界膜的血管内皮细胞核,经索拉非尼治疗后,突破内界膜的血管内皮细胞核明显减少,且呈剂量依赖性。A组、B组、C组、D组、E组、F组突破视网膜内界膜的血管内皮细胞核数分别为(0.22±0.42)个、(35.66±4.70)个、(35.30±4.54)个、(27.30±4.28)个、(21.41±3.53)个、(7.41±2.87)个;除B组与C组间差异无统计学意义外,其余各组间差异均有统计学意义(均为P0.05)。结论索拉非尼能够有效抑制大鼠OIR模型视网膜新生血管的生成,且抑制效果呈剂量依赖性。
[Abstract]:Objective to investigate the effect of intravitreous injection of Solafenil on retinal neovascularization in oxygen-induced retinopathy (oxygen-induced retinopathy,OIR) rats. Methods one hundred and forty-four SD neonatal rats aged 7 days were randomly divided into 6 groups: control group B: group C: ROP: control group: group D: ROP 5 渭 g, group E: group E, group E: ROP 20 渭 g, group F, group ROW ROP 80 渭 g, group D: Solafenil group. OIR model was established in all groups except group A in normal oxygen environment. The neonatal rats were given intravitreal injection of DMSO and the corresponding dose of Solafenib on the 12th day after birth, and then killed on the 17th day after birth. The retinal vascular morphology was observed under laser confocal microscope and the number of vascular branching points was counted in high power field. The retinal tissue morphology was observed by HE staining and the number of vascular endothelial nuclei breaking through the inner boundary membrane was counted. Results Retinal smears showed that retinal vessels in group B and C were dilated and formed a large number of neovascularization, and retinal neovascularization decreased significantly after treatment with Solafenib. In a dose-dependent manner, there were (16.50 卤3.90), (37.44 卤6.47), (37.08 卤5.10), (30.80 卤6.85), (26.08 卤5.08), (19.83 卤3.51) vascular branches in group E, (37.44 卤6.47), (30.80 卤6.85), (26.08 卤5.08), (19.83 卤3.51), respectively. There were significant differences among the other groups (P0.05). The HE staining showed that there were a large number of vascular endothelial nuclei in group B and group C which broke through the inner boundary membrane. After treatment with Solafenil, the number of endothelial nuclei breaking through the inner boundary membrane decreased significantly. In a dose-dependent manner, the number of vascular endothelial nuclei in group A, group B, group C, group D, and group E, respectively, were (0.22 卤0.42), (35.66 卤4.70), (35.30 卤4.54), (27.30 卤4.28), (21.41 卤3.53), (7.41 卤2.87) respectively, except that there was no significant difference between group B and group C. There were significant differences among the other groups (P0.05). Conclusion Solafenil can effectively inhibit retinal neovascularization in rat OIR model in a dose-dependent manner.
【作者单位】：石河子大学医学院;解放军第474医院全军眼科中心;山西省运城市中心医院眼科;
【分类号】：R774.1;R722.6

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