锌指转录因子Snail在喉癌中的表达及其与不同亚群喉癌细胞侵袭和转移的相关性研究

发布时间：2018-09-17 20:31

【摘要】：目的探讨锌指转录因子Snail在喉鳞状细胞癌中的表达情况及其与临床特征的关系,通过检测不同喉癌细胞亚群中Snail的表达研究其与喉癌侵袭转移的相关性。方法免疫组化法检测吉林大学第二医院耳鼻咽喉头颈外科48例喉癌组织和20例癌旁对照组织手术标本Snail蛋白的表达情况,并分析Snail与临床特征的相关性。采用RT—PCR及Western blot和细胞免疫化学染色检测CD 44~+CD 133~+、CD 44~-和CD 133~-不同亚群喉癌细胞中Snail和粘附分子E—cadherin的表达情况,并与侵袭及转移进行相关性探讨。结果 Snail在喉癌组织中表达水平显著高于癌旁对照组织(P0.01)。Snail的表达与喉癌的临床分期、淋巴结转移、肿瘤分化程度密切相关(P0.05),而与患者的性别、年龄、临床分型等无关。RT—PCR及Western blot检测结果均证实,Snail在CD 44~+CD 133~+喉癌细胞中的表达水平明显高于CD 44~-和CD 1333~-喉癌细胞,而粘附分子E—cadherin在CD 44~+CD 133~+喉癌细胞中的表达水平明显低于CD 44~-和CD 133~-喉癌细胞,细胞免疫组化染色证实CD 44~+CD 133~+喉癌亚群细胞中Snail和E—cadherin的表达呈负相关。结论 Snail的表达与喉癌的侵袭转移密切相关,且在高致瘤亚群CD 44~+CD 133~+喉癌细胞中呈显著高表达,与粘附分子E—cadherin的表达呈负相关,与喉癌细胞的侵袭和转移具有密切相关性。
[Abstract]:Objective to investigate the expression of zinc finger transcription factor (Snail) in laryngeal squamous cell carcinoma (LSCC) and its relationship with clinical features, and to investigate the relationship between the expression of Snail and invasion and metastasis of laryngeal carcinoma by detecting the expression of Snail in different subsets of laryngeal carcinoma. Methods Immunohistochemical method was used to detect the expression of Snail protein in 48 cases of laryngeal carcinoma and 20 cases of adjacent control tissue in otolaryngopharyngeal head and neck surgery of Jilin University, and the correlation between Snail and clinical features was analyzed. The expression of Snail and adhesion molecule E-cadherin was detected by RT-PCR, Western blot and cellular immunocytochemical staining in CD 44 ~ CD 133 ~ + CD44- and CD 133- subsets of laryngeal cancer cells, and the correlation with invasion and metastasis was discussed. Results the expression of Snail in laryngeal carcinoma tissues was significantly higher than that in the adjacent control tissues (P0.01). Snail expression was closely related to the clinical stage, lymph node metastasis and tumor differentiation degree of laryngeal carcinoma (P0.05), but it was related to the sex and age of the patients. The results of RT-PCR and Western blot showed that the expression of snail in CD 44 ~ CD 133 ~ (-) laryngeal carcinoma cells was significantly higher than that in CD 44 ~ (-) and CD 1333 ~ (-) laryngeal carcinoma cells. The expression of adhesion molecule E-cadherin in CD 44 ~ CD 133 ~ (-) laryngeal carcinoma cells was significantly lower than that in CD 44-and CD 133 ~ (-) laryngeal carcinoma cells. The expression of Snail and E-cadherin in CD 44 ~ CD 133 ~ laryngeal carcinoma subgroup cells was negatively correlated with immunohistochemical staining. Conclusion the expression of Snail is closely related to the invasion and metastasis of laryngeal carcinoma, and is highly expressed in the high tumor subgroup CD 44 ~ CD 133 ~, and negatively correlated with the expression of adhesion molecule E-cadherin. It is closely related to the invasion and metastasis of laryngeal cancer cells.
【作者单位】：吉林大学第二临床医院耳鼻咽喉头颈外科;
【基金】：吉林省自然科学基金项目(20130101151JC) 教育部博士点新教师基金项目(20120061120092)
【分类号】：R739.65

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