黄芪多糖对视网膜母细胞瘤细胞侵袭能力的影响

发布时间：2018-10-10 06:21

【摘要】：目的探讨黄芪多糖(astragalus polydsaccharide,APS)对视网膜母细胞瘤RB44细胞侵袭能力的影响及机制。方法以200 mg·L-1APS诱导48 h的RB44细胞为实验组(预实验结果),以不加药的RB44细胞为对照组。通过Transwell迁移和侵袭实验观察APS对RB44细胞迁移和侵袭能力的影响,通过Western blotting方法检测APS处理前后RB44细胞中侵袭相关基因蛋白表达的变化。结果 Transwell迁移和侵袭实验结果显示:与对照组每高倍视野(54.667±4.041)个和(26.000±2.646)个细胞相比,APS组RB44细胞穿过Transwell小室基膜的细胞数量显著减少,分别为每高倍视野(26.667±4.041)个和(8.667±2.082)个细胞(均为P0.01)。Western blotting结果显示:与对照组(0.128±0.019)相比,APS组RB44细胞中E-cadherin的表达(0.117±0.019)差异无统计学意义(P=0.486),而MMP-9和MMP-2的表达(0.134±0.008、0.085±0.006)与对照组(0.269±0.187、0.223±0.013)相比均显著降低,差异均有显著统计学意义(均为P0.01)。结论 APS可显著抑制视网膜母细胞瘤RB44细胞的侵袭能力,其机制可能与抑制MMP-9和MMP-2的表达有关。
[Abstract]:Objective to investigate the effect and mechanism of astragalus polysaccharide (astragalus polydsaccharide,APS) on the invasiveness of RB44 cells in retinoblastoma. Methods RB44 cells were induced for 48 h with 200 mg L-1APS as experimental group (pretest results) and RB44 cells without drug as control group. The effect of APS on the migration and invasion of RB44 cells was observed by Transwell migration and invasion assay. The expression of invasive-associated gene protein in RB44 cells was detected by Western blotting method before and after APS treatment. Results the results of Transwell migration and invasion test showed that compared with the control group, the number of RB44 cells passing through the basement membrane of Transwell chamber was significantly decreased compared with that of the control group (54.667 卤4.041) and (26.000 卤2.646) cells. The results of Western blotting showed that there was no significant difference in the expression of E-cadherin in RB44 cells compared with the control group (0.128 卤0.019) (P > 0.486), but the expression of MMP-9 and MMP-2 (0.134 卤0.0080.085 卤0.006) was significantly lower than that in the control group (0.269 卤0.187 卤0.223 卤0.013). The difference was statistically significant (all P 0.01). Conclusion APS can significantly inhibit the invasiveness of RB44 cells in retinoblastoma, and its mechanism may be related to the inhibition of MMP-9 and MMP-2 expression.
【作者单位】：南方医科大学南方医院新生儿科;新乡医学院病理学教研室;
【基金】：河南省基础与前沿技术研究计划(编号:122300410121)~~
【分类号】：R739.7

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