鼻咽癌淋巴结转移相关分子蛋白质组学技术筛选与鉴定

发布时间：2018-10-19 16:30

【摘要】：目的:比较2组不同程度颈部淋巴节结转移的初诊鼻咽癌组织在蛋白质组水平上的表达差异。方法:将52例2010-06-01-2011-03-31中山大学肿瘤防治中心初诊鼻咽癌患者,按照颈部淋巴结转移程度分为A组(N0~N1)和B组(N2~N3),分别收集每组患者的肿瘤组织,提取总蛋白,进行双向凝胶电泳分离和质谱分析,利用UMAX PowerLook1100透射扫描仪获取凝胶图像,应用PDQuest 7.1.0软件包进行图像分析,选取凝胶图谱中差异表达1.5倍且P0.01的蛋白质斑点,应用基质辅助激光解吸/电离-飞行时间质谱仪(matrix assisted laser desorption ionization time of flight/mass spectrometry,MALDI-TOF/MS)进行蛋白质的肽指纹图谱鉴定。结果:在2组颈部淋巴节结转移程度不同的初诊鼻咽癌患者肿瘤组织间,共有7个蛋白质点强度差异≥1.5倍且P0.01。经质谱分析鉴定发现,B组中的表达量比在A组中的表达量,Stathmin升高5倍,缺氧诱导因子1-α抑制剂升高10倍。结论:Stathmin和缺氧诱导因子1-α抑制剂在高淋巴结转移的鼻咽癌组织中表达增加,提示其在肿瘤发生发展机制中的作用可能与淋巴结转移有关。
[Abstract]:Aim: to compare the proteomic expression of nasopharyngeal carcinoma (NPC) with different degrees of cervical lymph node metastasis. Methods: 52 patients with nasopharyngeal carcinoma (NPC) at Cancer Prevention Center of Sun Yat-sen University, 2010-06-01-2011-03-31 were divided into two groups according to the degree of cervical lymph node metastasis: group A (N0~N1) and group B (N2~N3). The tumor tissues of each group were collected and the total protein was extracted. Two-dimensional gel electrophoresis separation and mass spectrometry analysis were performed. The gel images were obtained by UMAX PowerLook1100 transmission scanner and analyzed by PDQuest 7.1.0 software package. Protein spots with 1.5 times differential expression and P0.01 were selected. Matrix assisted laser desorption / ionization-time of flight mass spectrometer (matrix assisted laser desorption ionization time of flight/mass spectrometry,MALDI-TOF/MS) was used to identify the peptide fingerprint of protein. Results: in two groups of nasopharyngeal carcinoma patients with different degree of cervical lymph node metastasis, there were 7 protein spots with a intensity of more than 1.5 times and P0.01. The expression of Stathmin in group B was 5 times higher than that in group A, and the expression of hypoxia inducible factor 1- 伪 inhibitor was 10 times higher than that in group A. Conclusion: the expression of Stathmin and hypoxia inducible factor 1- 伪 inhibitor is increased in nasopharyngeal carcinoma with high lymph node metastasis, which suggests that the expression of Stathmin and hypoxia inducible factor 1- 伪 inhibitor may be related to lymph node metastasis.
【作者单位】：中山大学附属中山医院放疗科;中山大学肿瘤防治中心肿瘤研究所·华南肿瘤学国家重点实验室;
【基金】：国家十一五科技支撑计划(2006BAI02A11)
【分类号】：R739.63

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