豚鼠耳蜗三维重建与基底膜模态数值模拟的研究

发布时间：2018-11-17 18:59

【摘要】：目的: 1.基于组织的连续切片并结合有限元软件实现耳蜗的三维重建,使得宏观解剖结构得到清晰的显示,在阐明生物体结构和功能的互动机制及形态学、解剖学等领域的研究具有重要的意义。 2.通过耳蜗的三维重建,不但能精确显示耳蜗复杂的解剖结构,并可进行多个视角的旋转,获得长度、面积、体积和角度等精准的解剖参数,为内耳相关疾病的病理生理及发病机制等基础研究、也为临床选择最佳手术路径及电子耳蜗的研制和植入提供了量化的分析平台。 3.通过耳蜗的三维重建,使我们更加直观的了解耳蜗与毗邻结构甚至与头颅的相对位置关系,同时也为进一步实现其它组织器官局部乃至整体的三维重建提供了方法参考。 4.基于耳蜗三维重建,利用有限元专用软件,建立耳蜗基底膜数值模型。探讨了耳蜗基底膜的结构特征并进行模态分析,为内耳功能的数值模拟提供了研究基础。材料及方法:取成年健康豚鼠1只,行电耳镜、听性脑干反应、耳声发射及内耳薄层CT扫描,除外耳部疾患及内耳畸形,全麻后迅速断头取出左侧颞骨,打开听泡,暴露耳蜗,并灌注及固定,经修剪、脱钙、脱水、透明、浸蜡、包埋、定位等步骤后行石蜡连续切片及排序,切片厚度0.6×10-5 m,固定后染色、封片。对切片进行连续摄像,获得一整套豚鼠内耳组织切片的图像资料,将图像导入Photoshop软件,进行去色、调整灰度及对比度等处理后,利用mimics软件识别边界并标定,获得边界点的三维坐标,将坐标导入Hypermesh软件建立网格化模型,利用ANSYS有限元软件进行重建,得到豚鼠耳蜗膜结构的三维有限元模型,应用有限元方法,求解基底膜简化模型的模态。结果: 1.通过组织连续石蜡切片及摄像,获取了一整套豚鼠耳蜗二维连续切片的图像信息。 2.利用石蜡连续切片并结合有限元软件,建立了豚鼠耳蜗的三维有限元模型,该模型清晰地显示耳蜗的空间结构特性,具有实际意义及应用价值。 3.建立了基底膜三维重建模型,利用ANSYS软件求解基底膜的模态,获得了了豚鼠基底膜的前十阶模态。结论: 1.基于组织连续切片的计算机三维重建,既具备切片的细节性,又能反映其解剖结构的空间特性,是一种提高耳蜗和颞骨形态学研究水平的方法。 2.通过组织连续石蜡切片技术并结合ANSYS软件,能够实现耳蜗的三维重建,为耳蜗内相关结构与功能的互动机制数值模型的研究提供基础。 3.依据耳蜗基底膜固有的动力学属性,结合组织连续切片及ANSYS软件,建立了耳蜗基底膜的三维重建模型,通过求解模态,加深了对基底膜在听觉传导过程中作用机制的理解,为内耳疾病的研究提供了量化分析平台。
[Abstract]:Objective: 1. The three-dimensional reconstruction of cochlea based on tissue serial slice and finite element software makes the macroscopic anatomical structure clearly displayed, and the interactive mechanism and morphology of organism structure and function are clarified. The study of anatomy and other fields is of great significance. 2. Through the three-dimensional reconstruction of the cochlea, not only the complex anatomical structure of the cochlea can be accurately displayed, but also the multiple angles of view can be rotated, and the exact anatomical parameters such as length, area, volume and angle can be obtained. It provides a quantitative analysis platform for the basic research of pathophysiology and pathogenesis of inner ear diseases, and also provides a quantitative analysis platform for clinical selection of optimal surgical path and cochlear implant. 3. Through the three-dimensional reconstruction of cochlea, we can understand the relative position of cochlea and adjoining structure or even the head directly, and at the same time, it provides a method reference for the further realization of 3D reconstruction of other tissues and organs. 4. Based on three-dimensional reconstruction of cochlea, a numerical model of basal membrane of cochlea was established by using finite element software. The structural characteristics of the basal membrane of the cochlea were discussed and modal analysis was carried out, which provided a basis for the numerical simulation of the inner ear function. Materials and methods: a healthy adult guinea pig was treated with electroauroscope, auditory brainstem response, otoacoustic emission and thin layer CT scan of inner ear, excluding ear diseases and deformity of inner ear. After general anesthesia, left temporal bone was taken out quickly, auditory bubble was opened and cochlea was exposed. After pruning, decalcification, dehydration, transparency, wax immersion, embedding and positioning, paraffin sections were continuously sliced and sequenced. The thickness of sections was 0.6 脳 10 ~ (-5) m. A whole set of image data of guinea pig inner ear tissue slice was obtained by continuous camera. The image was imported into Photoshop software. After decolorization, gray scale and contrast adjustment were processed, the boundary was recognized and calibrated by mimics software. The 3D coordinates of the boundary point were obtained, the coordinates were imported into the Hypermesh software to establish the mesh model, and the ANSYS finite element software was used to reconstruct the 3D finite element model of the cochlea membrane structure of guinea pig, and the finite element method was used. The mode of simplified model of basement membrane is solved. Results: 1. A whole set of image information of 2-D continuous slice of guinea pig cochlea was obtained by tissue continuous paraffin section and camera. 2. A three-dimensional finite element model of guinea pig cochlea was established by using paraffin serial section and finite element software. The model clearly shows the spatial structure of the cochlea and has practical significance and application value. 3. The 3D reconstruction model of basement membrane was established, and the first ten modes of guinea-pig basement membrane were obtained by using ANSYS software to solve the mode of basement membrane. Conclusion: 1. The computer 3D reconstruction based on tissue serial section has not only the detail of the slice but also the spatial characteristics of its anatomical structure. It is a method to improve the research level of the morphology of cochlea and temporal bone. 2. The 3D reconstruction of cochlea can be realized by the technique of tissue continuous paraffin section and ANSYS software, which provides the basis for the study of the interactive mechanism of structure and function in cochlea. 3. According to the inherent dynamic properties of the basal membrane of cochlea, the 3D reconstruction model of the basal membrane of cochlea was established by combining with the tissue serial slice and ANSYS software. By solving the mode, the mechanism of the basement membrane acting on the auditory conduction process was further understood. It provides a quantitative analysis platform for the study of inner ear disease.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R764

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