NFBD1在鼻咽癌组织中的表达及其对鼻咽癌细胞生长、周期、裸鼠移植瘤生长的影响
发布时间：2018-12-10 07:34
【摘要】：目的：研究鼻咽癌（nasopharyngeal carcinoma，NPC）临床标本中NFBD1（nuclear factor with BRCT domains protein1）的表达水平及其临床意义，探讨NFBD1表达下调对鼻咽癌细胞生长、周期、裸鼠移植瘤生长速度的影响。 方法：IHC法测量鼻咽癌组织（40例）及慢性鼻咽炎组织(20例)中蛋白水平上NFBD1的表达，实时荧光定量PCR检测34例鼻咽癌组织和23例慢性鼻咽炎组织中NFBD1mRNA的表达水平，，并分析患者性别、年龄、颈淋巴结转移和临床分期与之的相关性；培养鼻咽癌细胞株CNE-1，逆转录病毒介导的siRNA感染CNE-1细胞后，通过实时荧光定量PCR及免疫印迹（Western blot）检测NFBD1的表达水平变化，采用CCK-8法检测NFBD1siRNA对CNE-1细胞生长速度的改变，流式细胞术检测NFBD1siRNA对细胞周期的影响，并观察其对裸鼠皮下移植瘤生长速度的影响。 结果：鼻咽癌组织中NFBD1在蛋白水平(x2=9.600,P=0.002）和mRNA水平（t=3.206，P=0.002）表达均显著高于慢性鼻咽炎组织,NFBD1在mRNA水平的表达高低与患者的性别、年龄及是否伴有临床颈淋巴结转移、临床分期均没有相关性。NFBD1siRNA可以下调NFBD1在mRNA（t=22.517，P=0.002）及蛋白水平的表达，并可以抑制CNE-1细胞的生长增殖速度、阻滞细胞于细胞周期的G2M期，抑制CNE-1裸鼠皮下移植瘤的生长速度（t=5.563，P=0.003）。 结论：NFBD1在鼻咽癌组织中的表达高于慢性鼻咽炎组织，抑制NFBD1表达可以抑制细胞生长速度、改变细胞周期、降低裸鼠移植瘤生长速度，其可能参与了鼻咽癌的发生，促进其生长，但其与鼻咽癌的侵袭转移没有明显联系。
[Abstract]:Objective: to study the expression of NFBD1 (nuclear factor with BRCT domains protein1 in clinical specimens of nasopharyngeal carcinoma (nasopharyngeal carcinoma,NPC) and its clinical significance, and to explore the effect of down-regulation of NFBD1 expression on the growth, cycle and growth rate of nude mice xenografts. Methods: the expression of NFBD1 was measured by IHC in 40 cases of nasopharyngeal carcinoma (NPC) and 20 cases of chronic nasopharyngitis, and the expression of NFBD1mRNA in 34 cases of nasopharyngeal carcinoma and 23 cases of chronic nasopharyngitis was detected by real-time fluorescence quantitative PCR. The correlation between sex, age, cervical lymph node metastasis and clinical stage was analyzed. After CNE-1 cells were infected with siRNA mediated by CNE-1, retrovirus, the expression of NFBD1 was detected by real-time fluorescence quantitative PCR and Western blot (Western blot). CCK-8 assay was used to detect the change of growth rate of CNE-1 cells induced by NFBD1siRNA, and flow cytometry was used to detect the effect of NFBD1siRNA on cell cycle, and to observe the effect of NFBD1siRNA on the growth rate of subcutaneous transplanted tumor in nude mice. Results: the expression of NFBD1 and mRNA in nasopharyngeal carcinoma was significantly higher than that in chronic nasopharyngitis. The expression of NFBD1 in mRNA was significantly higher than that in chronic nasopharyngitis. There was no correlation between age and clinical cervical lymph node metastasis. NFBD1siRNA could down-regulate the expression of NFBD1 in mRNA and protein levels, and inhibit the growth and proliferation of CNE-1 cells. The growth rate of subcutaneous transplanted tumor in CNE-1 nude mice was inhibited by blocking the cell cycle in G 2m phase (t = 5.563P0. 003). Conclusion: the expression of NFBD1 in nasopharyngeal carcinoma is higher than that in chronic nasopharyngitis. Inhibition of NFBD1 expression can inhibit cell growth, change cell cycle and decrease growth rate of transplanted tumor in nude mice, which may be involved in the occurrence of nasopharyngeal carcinoma. Promote its growth, but it has no obvious relationship with invasion and metastasis of nasopharyngeal carcinoma.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R739.63
本文编号：2370206
[Abstract]:Objective: to study the expression of NFBD1 (nuclear factor with BRCT domains protein1 in clinical specimens of nasopharyngeal carcinoma (nasopharyngeal carcinoma,NPC) and its clinical significance, and to explore the effect of down-regulation of NFBD1 expression on the growth, cycle and growth rate of nude mice xenografts. Methods: the expression of NFBD1 was measured by IHC in 40 cases of nasopharyngeal carcinoma (NPC) and 20 cases of chronic nasopharyngitis, and the expression of NFBD1mRNA in 34 cases of nasopharyngeal carcinoma and 23 cases of chronic nasopharyngitis was detected by real-time fluorescence quantitative PCR. The correlation between sex, age, cervical lymph node metastasis and clinical stage was analyzed. After CNE-1 cells were infected with siRNA mediated by CNE-1, retrovirus, the expression of NFBD1 was detected by real-time fluorescence quantitative PCR and Western blot (Western blot). CCK-8 assay was used to detect the change of growth rate of CNE-1 cells induced by NFBD1siRNA, and flow cytometry was used to detect the effect of NFBD1siRNA on cell cycle, and to observe the effect of NFBD1siRNA on the growth rate of subcutaneous transplanted tumor in nude mice. Results: the expression of NFBD1 and mRNA in nasopharyngeal carcinoma was significantly higher than that in chronic nasopharyngitis. The expression of NFBD1 in mRNA was significantly higher than that in chronic nasopharyngitis. There was no correlation between age and clinical cervical lymph node metastasis. NFBD1siRNA could down-regulate the expression of NFBD1 in mRNA and protein levels, and inhibit the growth and proliferation of CNE-1 cells. The growth rate of subcutaneous transplanted tumor in CNE-1 nude mice was inhibited by blocking the cell cycle in G 2m phase (t = 5.563P0. 003). Conclusion: the expression of NFBD1 in nasopharyngeal carcinoma is higher than that in chronic nasopharyngitis. Inhibition of NFBD1 expression can inhibit cell growth, change cell cycle and decrease growth rate of transplanted tumor in nude mice, which may be involved in the occurrence of nasopharyngeal carcinoma. Promote its growth, but it has no obvious relationship with invasion and metastasis of nasopharyngeal carcinoma.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R739.63
【共引文献】
相关博士学位论文 前3条
1 刘峰;鼻咽癌残存病灶分次立体定向放射治疗临床研究[D];北京协和医学院;2013年
2 陈宇;CTMP在七氟醚预处理神经保护中的作用机制研究[D];第四军医大学;2013年
3 陈亮;miR-421下调FOXO4促进鼻咽癌细胞增殖及凋亡抵抗[D];南方医科大学;2013年
相关硕士学位论文 前5条
1 周杰;马来酰亚胺聚谷氨酸天冬氨酸靶向肽聚合物药物载体系统的建立与应用[D];华东师范大学;2013年
2 刘凯;多聚L-谷氨酸-γ-苄酯纳米粒子提高耐药细胞药物累积的研究[D];华东师范大学;2013年
3 阮开安;鼻咽癌放疗后局部复发的影像学分析[D];广西医科大学;2013年
4 林庆良;转移性鼻咽癌化疗方案的临床研究[D];福建医科大学;2013年
5 黄新义;siRNA沉默Survivin对鼻咽癌细胞CNE2的影响[D];南华大学;2013年
本文编号：2370206
本文链接：https://www.wllwen.com/yixuelunwen/yank/2370206.html
最近更新
教材专著
