多西他赛对兔眼碱烧伤角膜新生血管抑制作用机制的实验研究

发布时间：2019-05-06 19:06

【摘要】：目的：观察多西他赛对碱烧伤兔角膜新生血管化的抑制作用及角膜组织中血管内皮生长因子(vascular endothelium growth factor VEGF)的表达影响，评价多西他赛对兔碱烧伤后角膜新生血管是否具有抑制作用并初步探讨其作用机理，，旨在为该药的临床应用提供实验依据,为将来药物治疗CNV提供一条新途径。方法：新西兰健康白兔24只，均由南昌大学实验动物科学部提供,体质量1.5~2.0kg,雌雄兼有;双眼均为实验眼。碱烧伤后随机将24只兔分为对照组和3个实验组，选择多西他赛滴眼液药物质量浓度为A组10ug·ml~(-1)；B组20u g·ml~(-1)；c组40u g·ml~(-1)，每组6只12眼，对照组D组滴NS眼液每日3次作为阴性对照，共观察14d。所有动物均于造型后1d开始，给予氧氟沙星滴眼液点眼每日4次，连点三日。采用裂隙灯显微镜下观察并测量新生血管长度以计算新生血管化面积、记录新生血管数，应用免疫组织化学染色及RT-PCR检测血管内皮细胞生长因子(Vascular endothelial growth factor,VEGF)表达等方法，研究角膜新生血管的形成及抑制情况。结果：（1）观察结果：治疗组A、B、C组新生血管发生较迟、生长缓慢、新生血管化面积小，与对照组比较，在统计学上差异有显著性（P0.05），治疗组B、C组兔角膜新生血管面积低于治疗组A和对照组，在统计学上差异有显著性（P0.05）；（2）免疫组化染色显示治疗组B、C组VEGF的表达较治疗组A和对照组明显减少，RT-PCR角膜组织中VEGF mRNA在造模后各时间点的相对表达量，治疗组B、C组低于治疗组A和对照组，差异有统计学意义（P0.05）结论：多西他赛滴眼液可有效抑制碱烧伤兔角膜新生血管的增殖，并可减少角膜组织VEGF的表达，抑制效果具有浓度相关性。
[Abstract]:Objective: to observe the inhibitory effect of docetaxel on corneal neovascularization and the expression of vascular endothelial growth factor (VEGF) (vascular endothelium growth factor VEGF) in corneal tissue after alkali burn in rabbits. To evaluate the inhibitory effect of docetaxel on corneal neovascularization after alkali burn in rabbits and to explore its mechanism in order to provide experimental basis for the clinical application of docetaxel and provide a new way for future drug treatment of CNV. Methods: 24 healthy New Zealand white rabbits were provided by the Department of Experimental Animal Science, Nanchang University, with a body mass of 1.5 to 2.0 kg, both male and female, and both eyes were experimental eyes. After alkali burn, 24 rabbits were randomly divided into control group and three experimental groups. The concentration of docetaxel eye drops was 20 u g 路ml ~ (- 1) in 10ug 路ml ~ (- 1); B group. Group C (40 u g 路ml ~ (- 1), 6 eyes in each group (n = 12) and control group D (n = 6) were treated with NS eye drops 3 times a day as negative control for 14 days. All animals were given ofloxacin eye drops 4 times a day for 3 days. The length of neovascularization was observed and measured under slit lamp microscope to calculate the area of neovascularization, and the number of neovascularization was recorded. The (Vascular endothelial growth factor, of vascular endothelial growth factor (VEGF) was detected by immunohistochemical staining and RT-PCR. The expression of VEGF was used to study the formation and inhibition of corneal neovascularization. Results: (1) the results of observation: the neovascularization of treatment group A, B, C was late, the growth was slow, and the area of neovascularization was small. Compared with the control group, there was significant difference between the two groups (P0.05). The corneal neovascularization area in group C was significantly lower than that in group A and control group (P0.05). (2) Immunohistochemical staining showed that the expression of VEGF in treatment group B, C group was significantly lower than that in treatment group A and control group, and the relative expression of VEGF mRNA in RT-PCR corneal tissue at different time points after modeling, and treatment group B, C group. The difference between group C and control group was statistically significant (P0.05) conclusion: docetaxel eye drops can effectively inhibit the proliferation of corneal neovascularization and reduce the expression of VEGF in corneal tissue of alkali burned rabbits. The inhibitory effect was concentration dependent.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R779.1

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