小胶质细胞在视网膜母细胞瘤中的活化及分布研究

发布时间：2019-07-25 17:43

【摘要】：目的对人视网膜母细胞瘤标本中小胶质细胞的活跃性及其与肿瘤分化程度、肿瘤侵袭性的相关性进行分析,了解小胶质细胞在视网膜母细胞瘤中的活化程度和分布特点。方法共收集32例33眼人视网膜母细胞瘤病例标本,总结其临床资料及肿瘤细胞分化特点。以MHCII作为小胶质细胞标志物、MAP-2为肿瘤细胞标志物进行免疫组织化l 学染色和免疫荧光双标染色。应用SPSS软件对数据进行χ2检验,分析小胶质细胞细胞分布与肿瘤分化情况及肿瘤侵犯情况的关系。结果在人视网膜母细胞瘤标本中,MHCII阳性细胞计数与肿瘤分化程度相关(OR=4.8,P=0.041)。在低分化肿瘤中,小胶质细胞数量相对较少;而中高分化肿瘤中,小胶质细胞数量较多。MHCII阳性细胞计数与肿瘤侵袭性及前期放化疗情况无相关性。表达MAP-2的肿瘤细胞不表达MHCII。结论小胶质细胞在视网膜母细胞瘤中是活化的,分布特征与肿瘤细胞的分化程度密切相关,其生物学意义和细胞机制有待于进一步研究来阐释。
[Abstract]:Objective to analyze the activity of microglia in human retinoblastoma and its correlation with tumor differentiation and tumor invasiveness, and to understand the activation degree and distribution of microglia in retinoblastoma. Methods 32 cases (33 eyes) of human retinoblastoma were collected and their clinical data and tumor cell differentiation characteristics were summarized. MHCII was used as microglia marker and MAP-2 as tumor cell marker for immunohistologic l staining and immunofluorescence double staining. SPSS software was used to analyze the relationship between the distribution of microglia and tumor differentiation and tumor invasion. Results the number of MHCII positive cells was correlated with the degree of tumor differentiation (OR=4.8,P=0.041). In poorly differentiated tumors, the number of microglia was relatively small, while in moderately and highly differentiated tumors, the number of microglia was higher. The count of MHCII positive cells was not correlated with tumor invasiveness and early radiotherapy and chemotherapy. Tumor cells expressing MAP-2 do not express MHCII. Conclusion microglia are activated in Retinoblastoma and their distribution is closely related to the degree of differentiation of tumor cells. Its biological significance and cellular mechanism need to be further studied.
【作者单位】：北京和睦家医院眼科;北京大学第三医院眼科;霍普金斯大学医学院威尔玛眼科研究所;
【分类号】：R739.7

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