芒果苷对DSS诱导的小鼠溃疡性结肠炎的预防作用及其抗炎机制初步研究

发布时间：2018-01-12 13:01

本文关键词：芒果苷对DSS诱导的小鼠溃疡性结肠炎的预防作用及其抗炎机制初步研究　出处：《天然产物研究与开发》2016年02期 　论文类型：期刊论文

【摘要】：本文研究芒果苷(MGF)对小鼠溃疡性结肠炎(UC)的预防作用及其作用机制。选用昆明种小鼠,用葡聚糖硫酸钠(DSS)复制UC模型。动物随机分成空白对照组、DSS模型对照组、柳氮磺胺吡啶(SASP)阳性对照组(900 mg/kg)、MGF组(300、150、75 mg/kg)、MGF固体分散体(MGF-SD)组(折合成MGF为300 mg/kg)。除空白对照组外,其他组小鼠自由饮用2.5%DSS水溶液造模,连续14 d,造模的同时灌胃给予相应药物干预。隔天进行疾病活动指数(DAI)评分。于第14 d处死小鼠,对结肠进行大体形态损伤指数(CMDI)评分,HE染色后进行结肠病理组织学指数(CHPI)评分,ELISA法检测结肠组织IL-1β、IL-4、IL-6和IL-10水平。结果显示,模型对照组小鼠DAI、CMDI和CHPI评分均显著增加(P0.05),结肠组织中IL-1β、IL-6水平显著升高(P0.05),IL-4、IL-10水平显著降低(P0.01)。MGF可显著改善UC小鼠的DAI、CMDI和CHPI评分。MGF组和MGFSD组均能显著降低结肠组织IL-1β水平(P0.05);MGF高剂量组和MGF-SD组显著降低结肠组织IL-6水平,但组间无显著差异(P0.05);各药物组对结肠组织IL-4和IL-10水平的影响与模型组比较均无显著性(P0.05)。表明MGF对DSS诱导的小鼠UC具有较好的预防作用,可能与下调结肠组织中IL-1β、IL-6水平有关,进而调节结肠组织中促炎因子与抗炎因子的平衡。提高MGF的溶解度不能提高其对UC的防治作用。
[Abstract]:The preventive effect of mangiferin MGFon on ulcerative colitis in mice and its mechanism were studied. Kunming mice were selected. UC model was established with dextran sodium sulfate (DSS). The mice were randomly divided into control group (DSS model control group) and salicylate sulfampyridine (SASP) positive control group (900 mg / kg). The MGF group was treated with MGF-SD (MGF = 300mg / kg), except for the blank control group. The mice in other groups were free to drink 2.5 DSS aqueous solution for 14 days. The mice were sacrificed on the 14th day, and the colonic gross morphological damage index (CMDI) was scored. After HE staining, the colonic histopathology index (CHPI) and Elisa were used to detect the levels of IL-6 and IL-10 in colonic tissues. In the model control group, the CMDI and CHPI scores of the model control group increased significantly, while the level of IL-1 尾 -IL-6 in colon tissue increased significantly (P 0.05) and the level of IL-4 in the colon was significantly increased. The level of IL-10 decreased significantly (P 0.01). MGF could significantly improve the DAI of UC mice. CMDI and CHPI scores. MGF group and MGFSD group could significantly reduce the IL-1 尾 level of colon tissue. The level of IL-6 in colon tissue was significantly decreased in MGF high dose group and MGF-SD group, but there was no significant difference between the two groups (P 0.05). The effects of each drug group on the levels of IL-4 and IL-10 in colonic tissue were not significantly different from those in the model group, indicating that MGF had a better preventive effect on DSS induced UC in mice. It may be related to down-regulating the level of IL-1 尾 -IL-6 in colon tissue, and then regulating the balance of proinflammatory factor and anti-inflammatory factor in colon tissue, but increasing the solubility of MGF can not improve its preventive and therapeutic effect on UC.
【作者单位】：广西中医药大学;中药制剂共性技术研发重点实验室;广西卫生职业技术学院;广西中医药大学第一附属医院;
【基金】：国家自然科学基金(81560674) 广西自然科学基金(2013GXNSFAA019124;2014GXNSFAA118198) 广西高校中药制剂共性技术研发重点实验室建设专项
【分类号】：R965
【正文快照】： 炎性肠病(Inflammatory bowel disease,IBD)是一种慢性炎症性疾病,可分为溃疡性结肠炎(Ulcer-ative colitis,UC)以及克隆氏病(Crohn’s disease,CD)。UC通常在城市化程度较高的欧美发达地区呈高发趋势,已被WHO列为现代难治病之一,其发病机制尚未完全阐明。目前临床治疗UC,除了

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