扎考比利对大鼠离体冠状动脉环的舒张作用及其机制

发布时间：2018-02-06 05:42

本文关键词： 扎考比利冠状动脉血管舒张内向整流钾通道　出处：《中国循环杂志》2015年12期 　论文类型：期刊论文

【摘要】：目的:研究扎考比利(zacopride)舒张大鼠冠状动脉环的作用及其可能机制。方法:雄性SD大鼠冠状动脉环的张力舒缩状态采用PowerLab和DMT系统记录,SD大鼠随机分为4组,内皮完整[+Endo,+Endo(vehicle)]组、+Endo(zacopride)组、完全去除内皮[-Endo,-Endo(vehicle)]组和-Endo(zacopride)组,每组6只大鼠,观察zacopride对KCl、血管收缩剂血栓素A2类似物(U46619)预收缩离体大鼠冠状动脉环的舒张作用;研究不同作用类药物对zacopride舒张血管作用的影响。结果:在+Endo(zacopride)组和-Endo(zacopride)组,Zacopride对KCl(60 mmol/L)和U46619(10~(-6)mol/L)预收缩的冠状动脉环呈现浓度依赖性地舒张作用,+Endo(zacopride)组Zacopride对KCl和U46619预收缩冠状动脉的最大舒张幅度分别为(90.15±6.38)%和(81.67±4.97)%,-Endo(zacopride)组zacopride对KCl和U46619预收缩冠状动脉的最大舒张幅度分别为(85.48±5.04)%和(79.65±3.51)%,两组zacopride对KCl和U46619预收缩冠状动脉的最大舒张幅度都显著高于其+Endo(vehicle)组和-Endo(vehicle)组,差异有统计学意义(P0.05);内向整流钾通道(I_(k1))阻断剂BaCl_2明显减弱zacopride的舒张血管作用,使zacopride对KCl和U46619预收缩动脉环的最大舒张幅度均减小(P0.05),差异有统计学意义。而一氧化氮合酶抑制剂亚硝基左旋精氨酸甲酯(L-NAME)、钙激活钾通道阻断剂四乙胺(TEA)、三磷酸腺苷敏感性钾通道阻断剂格列苯脲(Glib)和电压依赖性钾通道阻断剂4-氨基吡啶(4-AP)对zacopride的舒张血管作用没有明显影响(P均0.05)。结论:Zacopride对KCl和U46619预收缩大鼠冠状动脉环有明显的舒张作用;Zacopride对冠状动脉的舒张作用与激动I_(K1)通道有关。
[Abstract]:Objective: to study the zacopride of Zacopride. Methods: the tension and contraction of coronary rings in male SD rats were recorded by PowerLab and DMT system. SD rats were randomly divided into 4 groups with intact endothelium. [Endozacopride (Endozacopride) group, complete endothelial removal. [In each group, 6 rats in each group were treated with zacopride. The KCl was compared with that of the control group (n = 6) and the group of -Endozacopride (n = 6). Vasoconstrictor thromboxane A2 analogue U46619) relaxation of isolated rat coronary artery rings; Objective: to study the effect of different drugs on vasodilation of zacopride. Results: in the end of zacopride group and -Endozacopride group. Zacopride showed a concentration-dependent relaxation effect on KCl(60 mmol / L and U4661910 / L precontracted coronary rings. The maximal diastolic amplitude of Zacopride to KCl and U46619 precontracted coronary arteries were 90.15 卤6.38% and 90.15 卤6.38% respectively in the Endozacopride group. 81.67 卤4.97%. The maximal diastolic amplitude of zacopride to KCl and U46619 precontracted coronary arteries in the group of -Endozacopride was 85.48 卤5.04% and (. 79.65 卤3.51%. The maximal diastolic amplitude of zacopride to KCl and U46619 precontracted coronary arteries was significantly higher than that of KCl and U46619 groups. The vehicle group. The difference was statistically significant (P 0.05). BaCl_2, an inward rectifier potassium channel inhibitor, significantly reduced the vasodilation effect of zacopride. The maximal diastolic amplitude of zacopride to KCl and U46619 precontracted arterial rings was decreased (P 0.05). The difference was statistically significant. The nitric oxide synthase inhibitor, L-NAMEN, and the calcium activated potassium channel blocker, Tetraethylamine (tea). Adenosine triphosphate sensitive potassium channel blocker glibenclamide (Glib) and voltage-dependent potassium channel blocker 4-aminopyridine 4-AP). There was no significant effect on vasodilation of zacopride (P < 0.05). ConclusionZacopride has a significant relaxation effect on coronary annulus of KCl and U46619 precontract rats. The vasodilation effect of Zacopride on coronary artery is related to the activation of I _ S K _ 1) channel.
【作者单位】：山西医科大学人体解剖学教研室;
【基金】：山西省自然科学基金(2015021177) 山西医科大学博士启动基金(03201405);山西医科大学青年基金(Q02201205);山西医科大学基础医学院331培植项目(201208)
【分类号】：R965

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