环丙沙星和左氧氟沙星对豚鼠右心室乳头肌动作电位的影响

发布时间：2018-02-20 07:24

本文关键词： 环丙沙星左氧氟沙星右心室乳头肌动作电位豚鼠　出处：《郑州大学学报(医学版)》2017年01期 　论文类型：期刊论文

【摘要】：目的:观察环丙沙星(CIP)和左氧氟沙星(LVFX)对豚鼠右心室乳头肌动作电位的影响。方法:取豚鼠右心室乳头肌,用氧饱和的台式液持续表面灌流。采用累积浓度给药法于灌流液中加入CIP或LVFX,使药物终浓度依次达到0、1、10和100μmol/L。用标准微电极技术记录乳头肌加药前后静息电位(RP)、动作电位幅值(APA)、动作电位复极至50%的时程(APD50)及APD90。以司帕沙星(SPX)为阳性对照药。每组6个样本。结果:3个药物对RP、APA均没有影响(P0.05)。SPX可延长APD50和APD90,并且作用随浓度增加而增强(P0.001)。LVFX不延长APD50(P=0.110),可延长APD90,并且该作用随浓度增加而增强(P0.001)。CIP对APD50无影响,仅在100μmol/L时表现出延长APD90的作用(P=0.002)。结论:大剂量CIP和LVFX可引起豚鼠右心室乳头肌动作电位时程延长,具有潜在的心脏毒性。
[Abstract]:Objective: to observe the effects of ciprofloxacin (CIPP) and levofloxacin (LVFX) on the action potential of right ventricular papillary muscle in guinea pig. Continuous surface perfusion of oxygen-saturated tabletop solution was carried out. CIP or LVFX were added to perfusate by the method of cumulative concentration administration, and the final concentration of the drug reached 0 ~ 1 ~ 10 渭 mol / L and 100 渭 mol / L respectively. The resting potential of papillary muscle was recorded by standard microelectrode technique before and after addition of the drug. APD50 and APD90 were determined by the amplitudes of potential, action potential repolarization to 50% (APD50) and APD90.Sparfloxacin was used as a positive control drug. Six samples were collected from each group. Results: all three drugs had no effect on APD50 and APD90, and the effect increased with the increase of concentration. P0.001U. LVFX could prolong APD90 without prolonging APD50P0. 01, and the effect increased with the increase of concentration. CIP had no effect on APD50. Conclusion: high dose of CIP and LVFX can prolong the duration of action potential of right ventricular papillary muscle of guinea pig and have potential cardiac toxicity.
【作者单位】：河南医学高等专科学校基础医学部生理学教研室;
【基金】：河南省基础与前沿技术研究计划项目142300410469 河南省高等学校青年骨干教师资助计划项目2014GGJS-199
【分类号】：R965

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