雷帕霉素激活自噬流逆转脂多糖所致的急性肺损伤

发布时间：2018-03-05 11:06

  本文选题：脂多糖　切入点：脓毒症　出处：《中国药科大学学报》2015年05期 　论文类型：期刊论文

【摘要】：为探讨自噬对脂多糖(LPS)所致脓毒症小鼠急性肺损伤的影响,采用健康昆明小鼠随机分为空白组、模型组(LPS,10 mg/kg),雷帕霉素组(RAP,6 mg/kg),RAP-LPS组(RAP 6 mg/kg+LPS 10 mg/kg),3-甲基腺嘌呤组(3-MA,300μg/kg)和3-MA-LPS组(3-MA 300μg/kg+LPS 10 mg/kg),腹腔注射,给药6 h后,检测小鼠死亡率、肺组织中性粒细胞浸润、以及TNF-α、LC3和P62的表达。结果显示在LPS组中,小鼠肺组织中性粒细胞浸润显著增多,死亡率增加,TNF-α、LC3-Ⅱ和P62表达升高;RAP-LPS组较模型组死亡率下降,中性粒细胞浸润、P62和TNF-α均减少,而LC3-Ⅱ略升高;在3-MA-LPS组中,LC3-Ⅱ较LPS组降低,而中性粒细胞浸润、P62和TNF-α则没有差异。结果表明在LPS所致的脓毒症中,小鼠肺组织存在自噬流阻断,自噬体成熟障碍,RAP则能逆转LPS所致的肺组织自噬流障碍,改善细胞内环境,减轻炎性反应,降低死亡率。
[Abstract]:To investigate the effect of autophagy on acute lung injury in septic mice induced by lipopolysaccharide (LPS), healthy Kunming mice were randomly divided into blank group. Model group (10 mg / kg), rapamycin group (6 mg / kg), RAP-LPS group (10 mg / kg / kg) and 3-MA-300 渭 g / kg LPS (10 mg / kg) and 3-MA-LPS group (30 渭 g / kg LPS / kg / kg), intraperitoneal injection of rapamycin 6 mg / kg / kg / kg RAP-LPS group, lung neutrophil infiltration, lung tissue neutrophil infiltration, intraperitoneal injection of rapamycin 6 mg / kg / kg RAP-LPS group, lung tissue neutrophil infiltration, intraperitoneal injection of rapamycin 6 mg / kg / kg RAP-LPS group, neutrophil infiltration in lung tissue, The results showed that in LPS group, the neutrophil infiltration was significantly increased, the mortality increased, the expression of TNF- 伪 TNF- 伪 -LC3- 鈪，

本文编号：1569977