原料血浆和凝血因子类产品中人细小病毒B19污染情况调查

发布时间：2018-03-07 03:13

  本文选题：人细小病毒B　切入点：血浆　出处：《军事医学》2015年03期 　论文类型：期刊论文

【摘要】：目的检测混合原料血浆和凝血因子类产品中人细小病毒B19(human parvovirus B19,B19病毒)核酸,分析我国原料血浆及凝血因子类产品中B19病毒的污染情况。方法针对B19病毒的保守区域NS1区合成引物及探针,采用Taq Man实时定量PCR法检测混合原料血浆和4类凝血因子类产品中B19病毒核酸。结果混合原料血浆的B19病毒核酸的阳性率为59.49%(116/195),最高浓度可达1.35×1010拷贝/ml。因子Ⅷ、凝血酶、纤维蛋白原以及凝血酶原复合物的阳性率分别为93.55%(29/31)、100%(10/10)、85.71%(6/7)和88.89%(8/9)。结论我国混合原料血浆与凝血因子类产品中B19病毒的污染率较高,有必要进行原料血浆的B19病毒筛查,对于保障血液制品的病毒安全性具有重要意义。
[Abstract]:Objective to detect the nucleic acid of human parvovirus B19 parvovirus B19 virus in mixed raw plasma and coagulation factor products. The contamination of B19 virus in raw plasma and coagulation factor products in China was analyzed. Methods primers and probes were synthesized for the conserved NS1 region of B19 virus. Taq Man real-time quantitative PCR method was used to detect B19 virus nucleic acid in mixed raw plasma and four kinds of coagulation factor products. Results the positive rate of B19 virus nucleic acid in plasma of mixed raw material was 59.490.116 / 195. The highest concentration of B19 virus nucleic acid was 1.35 脳 10 10 copies / ml 路ml.Factor 鈪，

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