大鼠血浆中枸杞多糖荧光标记物定量分析方法的建立

发布时间：2018-03-16 17:33

  本文选题：异硫氰酸荧光素标记的枸杞多糖　切入点：药代动力学　出处：《食品科学》2015年02期 　论文类型：期刊论文

【摘要】：建立大鼠血浆中异硫氰酸荧光素标记的枸杞多糖(fluorescein isothiocyanate labeled Lycium barbarumpolysaccharides,LBP-FITC)的荧光定量分析方法,线性方程为y=1 378.7x-14.98(R2=0.998 9),血浆中LBP-FITC质量浓度在0.05~10μg/m L范围内与荧光强度呈良好的线性关系。方法的精密度、回收率以及稳定性考察分析证明该方法符合生物样品体内定量分析的要求。单次灌胃给予大鼠LBP-FITC后,以药代动力学Win Nonlin软件采用非房室模型拟合大鼠单次口服LBP-FITC的经时血药质量浓度数据,计算主要的药代动力学参数为最大峰质量浓度(Cmax)(2.39±0.71)μg/m L、达峰时间(tmax)(1.17±0.41)h;药时曲线下面积AUC0-t为(45.85±7.12)(μg?h)/m L,AUC0-∞为(127.31±39.00)(μg?h)/m L;半衰期(t1/2)为(80.32±32.70)h;清除率为(1 693.96±492.47)m L/kg;平均滞留时间为(20.64±1.36)h。
[Abstract]:A method for quantitative analysis of fluorescein isothiocyanate labeled Lycium barumPolysaccharide LBP-FITCin rat plasma with fluorescein isothiocyanate was established. The linear equation is YY 1 378.7x-14.98 9 渭 g / mL LBP-FITC concentration in plasma is linear with fluorescence intensity in the range of 0.05 ~ 10 渭 g / m ~ (-1). The precision of the method is obtained. The results of recovery and stability analysis showed that the method met the requirements of quantitative analysis of biological samples in vivo. After single oral administration of LBP-FITC in rats, The pharmacokinetic Win Nonlin software was used to fit the plasma drug concentration data of single oral LBP-FITC in rats with a non-atrioventricular model. The main pharmacokinetic parameters were calculated as the maximum peak concentration of C _ (max) 2.39 卤0.71) 渭 g / m ~ (L), the peak time was 1.17 卤0.41 h, and the area under the drug time curve was AUC0-t = 45.85 卤7.12 (渭 g 路L ~ (-1)). The value of AUC0- 鈭，

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