1株抗肿瘤银杏内生真菌的发酵条件及活性产物稳定性研究

发布时间：2018-03-29 16:19

  本文选题：银杏　切入点：内生真菌　出处：《江苏农业科学》2017年13期

【摘要】：对前期研究发现的1株分离自银杏树皮组织的具有良好的抗肿瘤活性内生真菌米曲霉菌(Aspergillus oryzae)YX-5菌株的发酵条件及活性产物稳定性进行研究。以发酵后的菌体干质量、粗提物质量和粗提物抗肿瘤活性为指标,对YX-5菌株的发酵温度、pH值、转速和发酵时间等条件进行优化。结果表明,最适宜的发酵条件为温度25℃、pH值7.0、摇床转速110 r/min、发酵时间7 d。以粗提物的抗肿瘤活性为指标,比较发酵液粗提物经热处理、酸碱处理、紫外照射和蛋白酶处理后的稳定性,发现YX-5菌株的活性产物在常温、中性和酸性环境中稳定性较好,对紫外线和蛋白酶有较强的抗性,但在超过100℃高温条件下或pH值超过9.0的碱性环境中稳定性较差。该菌株粗提物在自然条件下总体稳定性较高,不易发生变化,有利于活性成分分离工作的开展。
[Abstract]:The fermentation conditions and the stability of the active product of an endophytic fungus Aspergillus oryzae oryzae)YX-5, which was isolated from the bark of Ginkgo, were studied. The quality of crude extract and the antitumor activity of crude extract were used as indexes to optimize the fermentation temperature, pH value, rotational speed and fermentation time of YX-5 strain. The optimum fermentation conditions were as follows: temperature 25 鈩，

本文编号：1681897