依达拉奉对肝损伤模型小鼠的肝保护作用研究

发布时间：2018-04-18 20:21

本文选题：依达拉奉 + 对乙酰氨基酚　；参考：《中国药房》2015年13期

【摘要】：目的:研究依达拉奉对对乙酰氨基酚致肝损伤模型小鼠的保护作用。方法:取小鼠随机分为正常对照(生理盐水)组、模型(生理盐水)组、阳性对照(联苯双酯150 mg/kg)组和依达拉奉高、中、低剂量(10、5、2.5 mg/kg)组,每组10只,尾iv给予相应药物,连续14 d。末次给药后除正常对照组外,其余各组小鼠ip给予对乙酰氨基酚0.3 g/kg复制肝损伤模型。复制模型16 h后,各组小鼠眼球取血检测血清中谷氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、白细胞介素2(IL-2)、IL-6水平;处死小鼠后取肝组织,检测其中脂质过氧化物(LPO)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平并观察其病理学变化。结果:与正常对照组比较,模型组小鼠血清中ALT、AST、IL-6水平增加,IL-2含量降低,肝组织中LPO和MDA含量增加,SOD活性降低,差异均具有统计学意义(P0.01)。与模型组比较,依达拉奉高、中、低剂量组小鼠血清中ALT活性降低;依达拉奉高、中剂量组小鼠血清中IL-2含量增加,AST活性和IL-6含量降低,肝组织中LPO和MDA含量降低,SOD活性增强,差异均具有统计学意义(P0.05)。与模型组比较,依达拉奉高、中剂量组小鼠肝细胞变性、炎性细胞浸润减少,坏死程度显著减轻。结论:依达拉奉对对乙酰氨基酚致肝损伤模型小鼠有明显的保护作用,其机制可能与抗氧化及增强免疫有关。
[Abstract]:Aim: to study the protective effect of Edaravone on paracetamol induced liver injury in mice.Methods: mice were randomly divided into normal control group (normal saline) group, model group (normal saline) group, positive control group (150 mg / kg biphenyl diester) and Edaravone high, middle and low dose 1010 5 mg / kg group with 10 rats in each group.Continuous 14 days.After the last administration, the other mice were given acetaminophen 0.3 g/kg IP to establish the liver injury model except the normal control group.After 16 hours, the serum levels of glutamate aminotransferase (alt), aspartate aminotransferase (AST), interleukin-2 (IL 2) and interleukin 2 (IL 2) were detected in the blood of each group of mice, and the liver tissue was taken after the mice were killed.The levels of lipid peroxide (LPO), malondialdehyde (MDA) and superoxide dismutase (SOD) were measured and their pathological changes were observed.Results: compared with the normal control group, the serum level of LPO and MDA in the model group increased and decreased, while the content of LPO and MDA in the liver tissue increased and decreased. The difference was statistically significant (P 0.01).Compared with the model group, the activity of ALT in serum of Edaravone group was higher than that of the model group, and that of Edaravone group was higher than that of the model group, while the content of IL-2 and IL-6 in the middle dose group increased, and the content of LPO and MDA in liver tissue decreased.The difference was statistically significant (P 0.05).Compared with the model group, Edaravone was higher, liver cells denatured, inflammatory cell infiltration was decreased and necrosis degree was significantly reduced in middle dose group.Conclusion: Edaravone has obvious protective effect on paracetamol induced liver injury in mice, and its mechanism may be related to antioxidation and immune enhancement.
【作者单位】：安徽省妇幼保健院药学部;
【分类号】：R965

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