抗血栓苏云金芽孢杆菌的筛选及抗血栓机理的初步分析

发布时间：2018-05-09 00:31

本文选题：抗血栓 + 苏云金芽孢杆菌　；参考：《基因组学与应用生物学》2015年10期

【摘要】：我们利用VY/4平板法和酪蛋白平板法从水样、土样和朽木样品中筛选出6株可产生抗血栓活性物质的可滑动的细菌菌株,重点研究了菌株B4和B8,利用萃取法及(NH4)2SO4沉淀法对菌株B4和B8的胞外活性蛋白进行了初步分离。一方面,我们利用纤维蛋白平板法及加热的纤维蛋白平板法检测到菌株B4和B8的胞外蛋白可以通过直接降解纤维蛋白的方式溶解血栓,利用纤维蛋白原平板法及凝血酶平板法检测到菌株B4和B8的胞外蛋白可以通过降解纤维蛋白原的方式防止血栓的形成;另一方面,我们利用形态学特征、理化性质、16S r RNA序列同源性及系统进化树鉴定菌株B4和B8均属于苏云金芽孢杆菌(Bacillus thuringiensis)。综上所述,苏云金芽孢杆菌B4和B8的胞外蛋白既具有溶栓活性,又具有抗凝活性,即具有抗血栓的作用。
[Abstract]:Using VY/4 plate and casein plate method, we screened 6 slippable bacteria strains from water, soil and wood samples to produce antithrombotic active substances. The extracellular active proteins of strains B _ 4 and B _ 8 were isolated by extraction method and NH _ 4H _ 2SO _ 4 precipitation method. On the one hand, we used fibrin plate method and heated fibrin plate method to detect that the extracellular proteins of strain B4 and B8 can dissolve thrombus by directly degrading fibrin. The extracellular proteins of strains B4 and B8 were detected by fibrinogen plate method and thrombin plate method to prevent the formation of thrombus by degrading fibrinogen; on the other hand, we used morphological characteristics. The homology of 16s r RNA sequence and phylogenetic tree identified strains B4 and B8 belong to Bacillus thuringiensis. In conclusion, the extracellular proteins of Bacillus thuringiensis B4 and B8 have both thrombolytic activity and anticoagulant activity, that is, they have antithrombotic effect.
【作者单位】：成都大学临床医学院;成都大学基础医学与护理学院;成都大学生物工程学院;
【基金】：2014年国家级大学生创新创业训练计划项目(201411079004) 四川省教育厅重点项目(12ZA207)共同资助
【分类号】：R915

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